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Ultrastructural Localisation of TGF-β Exposure in Dentine by Chemical Treatment

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Abstract

Transforming growth factor-β (TGF-β) sequestered in dentine matrix has an important role in dental tissue repair after injury and its exposure at sites of injury may stimulate tertiary dentinogenesis. This study aimed to investigate the expression of TGF-β isoforms in mature human dentine matrix and the ability of chemical treatments to expose TGF-β on the cut surface of dentine using gold immunolabelling and subsequent scanning electron microscopy examination. TGF-β1 was the only isoform that could be detected in human dentine and the nature of the chemical treatment of the tissue influenced its detection. EDTA treatment provided good exposure of TGF-β1 on the dentine surface, whilst citric acid and sodium hypochlorite treatments revealed lesser amounts of this isoform. Only minimal staining for TGF-β1 was observed in samples treated with phosphate-buffered saline. TGF-β2 and -β3 could not be detected in the specimens with any of the treatments. This study suggests that TGF-β1 is the only TGF-β isoform expressed by human odontoblasts to be sequestered in dentine implying that differences in isoform–extracellular matrix interactions may exist. Information on chemical treatment of tissue specimens for immunostaining may provide a useful basis for selection of tissue preparation techniques for clinical restorative treatment procedures to facilitate TGF-β mediated reparative processes at sites of dental injury.

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Authors and Affiliations

  1. Conservative Dentistry, School of Stomatology, The Fourth Military Medical University, Xian, Peoples Republic of China

    S. Zhao

  2. Oral Biology, School of Dentistry, University of Birmingham, Birmingham, B4 6NN, UK

    A.J. Sloan, P.E. Murray, P.J. Lumley & A.J. Smith

Authors
  1. S. Zhao
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  2. A.J. Sloan
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  3. P.E. Murray
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  4. P.J. Lumley
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  5. A.J. Smith
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Zhao, S., Sloan, A., Murray, P. et al. Ultrastructural Localisation of TGF-β Exposure in Dentine by Chemical Treatment. Histochem J 32, 489–494 (2000). https://doi.org/10.1023/A:1004100518245

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