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Isozyme variation in Larrea ameghinoi and Larrea nitida (Zygophyllaceae): genetic diversity and its bearing on their relationship

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Abstract

Electrophoretic variants for 11 isozyme systems were examined by horizontal polyacrilamide gel electrophoresis in natural populations of Larrea ameghinoi and L. nitida (section Larrea). The two taxa presented fixed alternative allelic variants at loci Adh-1, Gdh-1 and Mdh-1. Genetic variability estimates showed no statistically significant differences among populations of section Larrea (He: 0.097–0.167). However, these values were significantly lower than those reported for species of section Bifolium (L. divaricata, diploid L. tridentata) in a previous contribution (He: 0.17–0.29). Positive and highly significant Wright's fixation indices are in agreement with higher amounts of inbreeding for nitida and L. ameghinoi, as suggested on the basis of floral morphology by previous authors. Moreover, the higher levels of genetic differentiation obtained for conspecific populations of section Larrea as compared to those of section Bifolium are also consistent with these observations. Nei's genetic identity values obtained for sympatric (I=0.80) and allopatric (I=0.63–0.73) L. ameghinoiL. nitida population pairs are concordant with those expected for different, but closely related congeneric species.

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References

  • Burdon, J.J., D.R. Marshall & R.H. Groves, 1980. Isozyme variation in Chondrilla juncea L. in Australia. Aust. J. Bot. 28: 193–198.

    Article  CAS  Google Scholar 

  • Cortés, M.C., 1987. Estudio de la variación alozímica en el género Larrea. Tesis Doctoral. Facultad de Cs. Exactas y Naturales, Universidad de Buenos Aires, 269 pp.

  • Cortés, M.C. & J.H. Hunziker, 1997. Isozymes in Larrea divaricata and Larrea tridentata (Zygophyllaceae): a study of two amphitropical vicariants and autopolyploidy. Genetica 101: 115–124.

    Article  PubMed  Google Scholar 

  • Crawford, D.J., 1990. Enzyme electrophoresis and plant systematics, pp. 146–164 in Isozymes in Plant Biology, edited by D.E. Soltis and P.S. Soltis. Chapman and Hall, London.

    Google Scholar 

  • Crawford, D.J. & R.J. Bayer, 1981. Allozyme divergence in Coreopsis ciclocarpa (Compositae). Syst. Bot. 6: 373–379.

    Article  Google Scholar 

  • Crawford, D.J. & E. Landolt, 1993. Allozyme studies in Spirodela (Lemnaceae): variation among conspecific clones and divergence among species. Syst. Bot. 18: 389–394.

    Article  Google Scholar 

  • Crawford, D.J., E. Landolt & D. H. Les, 1996. An allozyme study of two sibling species of Lemna (Lemnaceae) with comments on their morphology, ecology and distribution. Bull. Torrey Bot. Club. 123: 1–6.

    Article  Google Scholar 

  • Ezcurra, E., C. Montaña & S. Arizaga, 1991. Architecture, light interception and distribution of Larrea species in the Monte desert, Argentina. Ecology 72: 23–24.

    Article  Google Scholar 

  • Gottlieb, L.D., 1977. Electrophoretic evidence and plant systematics. Ann. Missouri. Bot. Gard. 64: 161–180.

    Article  Google Scholar 

  • Hamrick, J.L. & J.W. Godt, 1990. Allozyme diversity in plant species in Plant Population Genetics, breeding, and genetic resources, edited by H.D. Brown, M.T. Cleg, A.L. Kahler and B.S. Weir. Sinauer Associates Inc. Publishers Sunderland, Massachussetts.

    Google Scholar 

  • Hunziker, J.H., R.A. Palacios & A. Soriano, 1969. Hibridación natural en especies sudamericanas de Larrea (Zygophyllaceae). Kurtziana 5: 55–66.

    Google Scholar 

  • Hunziker, J.H., R.A. Palacios, L. Poggio, C.A. Naranjo & T.W. Yang, 1977. Geographic distribution, morphology, hybridization, cytogenetics and evolution, pp. 10–47 in Creosote Bush. Biology and Chemistry of Larrea in New World deserts, edited by T.J. Mabry, J.H. Hunziker and D.R. Difeo. US/IBP Synthesis Series N. 6, Dowden, Hutchinson and Ross, Inc.

  • Hunziker, J.H., R.A. Palacios, A.G. de Valesi & L. Poggio, 1978. Hybridization in Larrea (Zygophyllaceae): a morphological, cytogenetic, and chemosystematic study. Boletín Acad. Nac. Ciencias, Córdoba 52: 281–314.

    Google Scholar 

  • Jeffries, R.L. & L.D. Gottlieb, 1982. Genetic differentiation of the macrospecies Salicornia europaea (Sensu stricto) and S. ramosissima. J. Woods. New Phytol. 92: 123–129.

    Article  Google Scholar 

  • Manchenko, G.P., 1994. Handbook of Detection of Enzymes on Electrophoretic Gels. CRC Press Inc.

  • Nei, M., 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.

    Google Scholar 

  • Palacios, R.A. & J.H. Hunziker, 1972. Observaciones sobre la taxonomía del género Larrea (Zygophyllaceae). Darwiniana 17: 473–476.

    Google Scholar 

  • Panetta, F.D. & S.A. Carstairs, 1989. Isozymic discrimination of tropical Australian populations of mesquite (Prosopis spp.): implications for biological control. Weed Res. 29: 157–165.

    Google Scholar 

  • Poulik, M.D., 1957. Starch gel electrophoresis in a discontinuous system of buffers. Nature 180: 1477–1479.

    Article  PubMed  CAS  Google Scholar 

  • Ruiz Leal, A., 1970. Rehabilitación de Larrea simulans Sandwith (Zygophyllaceae). Boletin de la Sociedad Argentina de Botánica, 13: 182–187.

    Google Scholar 

  • Sandwith, N.Y., 1927. New species from the Andes of Argentina. Bull. Misc. Inform. 1: 174–178 (Royal Bot. Gard., Kew.).

    Google Scholar 

  • Scandalios, J.G., 1969. Genetic control of multiple molecular forms of isozymes in plants: a review. Biochem. Genet. 3: 37–79.

    Article  CAS  Google Scholar 

  • Schaal, B.A. & W.W. Anderson, 1974. An outline of techniques for starch gel electrophoresis of enzymes from the american oyster Crassostrea virginica Gmelin. Department of Zoology. Univ. Georgia, Athens, Georgia.

    Google Scholar 

  • Shaw, Ch.R. & R. Prasad, 1970. Starch gel electrophoresis of enzymes — a compilation of recipes. Biochem. Genet. 4: 279–320.

    Google Scholar 

  • Simpson, B.B., J.L. Neff & A.R. Moldenke, 1977. Reproductive systems of Larrea, pp. 92–114 in Creosote Bush. Biology and Chemistry of Larrea in New World Deserts, edited by T.J. Mabry, J.H. Hunziker and D.R. Difeo. US/IBP Synthesis Series N. 6, Dowden, Hutchinson and Ross, Inc.

  • Swofford, D.L. & R.B. Selander, 1981. Biosys-1: a FORTRAN program for the comprehensive analysis of electrophoretic data in population genetics and systematics. J. Heredity 72: 281–283.

    Google Scholar 

  • Thorpe, J.P., 1982. The molecular clock hypothesis: biochemical evolution, genetic differentiation, and systematics. Ann. Rev. Ecol. Syst. 13: 139–168.

    Article  CAS  Google Scholar 

  • Wendel, J.F. & N.F. Weeden, 1990. Visualization and interpretation of plant isozymes, pp. 5–45 in Isozymes in Plant Biology, edited by D.E. Soltis and P.S. Soltis. Chapman and Hall, London.

    Google Scholar 

  • Wright, S., 1931. Evolution in Mendelian populations. Genetics 16: 97–159.

    Google Scholar 

  • Wright, S., 1965. The interpretation of population structure by Fstatistics with special regard to systems of mating. Evolution 19: 395–420.

    Article  Google Scholar 

  • Wright, S., 1969. Evolution and the Genetics of Populations. Vol. 2: The Theory of Gene Frequencies. University of Chicago Press, Chicago.

    Google Scholar 

  • Wright, S., 1978. Evolution and the Genetics of Populations. Vol. 4: Variability Within and Among Natural Populations. University of Chicago Press. Chicago.

    Google Scholar 

  • Yang, T.W., J.H. Hunziker, L. Poggio & C.A. Naranjo, 1977. Hybridization between South American jarilla and North American diploid creosote bush (Larrea Cav., Zygophyllaceae). Plant Syst. Evol. 126: 331–346.

    Article  Google Scholar 

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Lia, V., Comas, C., Cortés, M. et al. Isozyme variation in Larrea ameghinoi and Larrea nitida (Zygophyllaceae): genetic diversity and its bearing on their relationship. Genetica 106, 197–207 (1999). https://doi.org/10.1023/A:1003973728628

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