Abstract
Two types of in vitro seedling tests were developed to evaluate resistance in flax (Linum usitatissimum) against Fusarium oxysporum f.sp. lini. In the first test a solid medium was used. The second test was based on a liquid medium. Disease severity was assessed after three weeks, using the observed reduction of plant length as a scale. Both methods proved to be useful for screening for resistance, for evaluating race specificity of resistance and for studying symptomatology. The solid medium method proved to be the more accurate for the screening, but the liquid medium method was much less time- and labour-consuming. The results of the tests were significantly correlated with observations under field conditions.
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References
Alabouvette C, Couteaudier Y and Louvet J (1982) A comparison of the receptivity of different soils and substrates to Fusariumwilts Comparaison de la réceptivité de différents sols et substrats de culture aux fusarioses vasculaires. Agronomie 2: 1-6
Anonymous (1986) Bulletin des variétés, plantes à fibres et oléagineuses, crucifères fourragères. Geves, La Minière: 27
Anonymous (1988) Bulletin des variétés, plantes à fibres et oléagineuses, crucifères fourragères. Geves, La Minière: 16
Anonymous (1990) Bulletin des variétés, plantes à fibres et oléagineuses, crucifères fourragères. Geves, La Minière: 13-23
Anonymous (1992) Bulletin des variétés, plantes à fibres et oléagineuses, crucifères fourragères. Geves, La Minière: 19-22
Anonymous (1994) Bulletin des variétés, plantes à fibres et oléagineuses, crucifères fourragères. Geves, La Minière: 16
Beaudoin X (1988) Disease and pest control. In: Marshall G (ed) Flax: Breeding and Utilisation. Kluwer Academic Publishers, Dordrecht: 81-88
Beaudoin X (1991) Testage de la résistance variétale à la fusariose. Activité Générale, Compte Rendu, Institut Technique Agricole du Lin 132: 1-4
Borlaug NE (1945) Variation and variability of Fusarium lini. University of Minnesota Agricultural Experiment Station. Technical Bulletin 168: 1-40
Bos L and Parlevliet JE (1995) Concepts and terminology on plant/pest relationships-toward consensus in plant pathology and crop protection. Annual Review of Phytopathology 33: 69-102
Broadfoot WC (1926) Studies on the parasitism of Fusarium liniBolley. Phytopathology 16: 951-978
Davis D (1966) Cross-protection in Fusariumwilt diseases. Phytopathology 57: 311-314
Ebskamp AGM and Bonthuis H (1993) List of cultivars of field crops 68, 68e Rassenlijst voor landbouwgewassen. De Boer, Hilversum: 60-64
Ebskamp AGM and Bonthuis H (1997) List of cultivars of field crops 72, 72e Rassenlijst voor landbouwgewassen De Boer, Hilversum: 52-57
Fouilloux G and Chaboche L (1996) Resistance of flax to Fusarium. Proceedings of the 4th European Regional Workshop on Flax, Rouen, France, September 25-28, 1996: 243-246
Gent RM (1995) —Untersuchungen zur Leinwelke (Fusarium oxysporumf. sp. lini(Bolley) Snyd. & Hans.) an Öllein (Linum usitatissimumL.) Research on Fusarium-wilt (Fusarium oxysporumf. sp. lini(Bolley) Snyd. & Hans.) in linseed (Linum usitatissimumL.). Selbstverlag; 121 pp. Bonn University (Germany). Universitaetsbibliothek
Jouan B and Sailly M (1991) —Compte Rendu d’activité du laboratoire de Rennes, Etude du Fusarium oxysporum. Activité Générale, Compte Rendu, Institut Technique Agricole du Lin 32: 1-17
Kenaschuk EO and Rashid KY (1993) Ac Linora flax. Canadian Journal of Plant Science 73: 839-841
Kenaschuk EO and Rashid KY (1994) Ac McDuff flax. Canadian Journal of Plant Science 74: 815-816
Kulkarni NB, More BB and Patil PL (1969) Occurrence of new race of Fusarium oxysporumSchl. f. lini(Bolley) Snyder & Hansen inciting linseed wilt in India. Mycopathologia et Mycologia Applicata. 38: 243-246
Li XP, Tian YJ, Li YH, Yin QZ and Guan XJ (1994) Brief report on the screening of flax varieties introduced from France. Crop Genetic Resources 4: 42
Liu XY, Chen SL, Sun QA, He DT and Wu YN (1993) Evaluation of Fusariumwilt resistance of flax varieties. Scientia Agricultura Sinica 26: 44-49
Löffler HJM, Straathof ThP, Van Rijbroek PCL and Roebroeck. EJA (1997) Fusariumresistance in Gladiolus: The development of a screening assay. Journal of Phytopathology 145: 465-468
Murashige T and Skoog F (1962) A revised medium for rapid assays with tobacco tissue cultures. Physiologica Plantarum 15: 473- 497
Ondrej M (1993) Evaluation of flax genepool according to resistance to Fusariumwilt of flax and to mildew. Plant Genetic Resources 92: 54-58
Popescu F (1995) Studies on inheritance of resistance to Fusariumwilt in linseed. Cercetari privind ereditatea rezistentei la fuzarioza a inului de ulei. Probleme de Genetica Teoretica si Aplicata 27: 37-56
Popescu F, Doucet I and Marinescu I (1994) Geria-the first Romanian linseed variety resistant to wilt (Fusarium oxysporumf. sp. lini(Bolley) Snyd. & Hans.). Romanian Agricultural Research 2: 77-81
Rashid KY and Kenaschuk EO (1993) Effect of trifluralin on Fusariumwilt in flax. Canadian Journal of Plant Science 73: 893-901
Tamietti G and Pramotton R (1987) A very simple method of selecting non-pathogenic isolates of Fusariumspp. antagonistic to vascular Fusariumdiseases. Une méthode très simple de sélection de souches de Fusariumspp. non pathogènes, antagonistes des fusarioses vasculaires. Bulletin OEPP 17: 549-557
Van Westrhenen KJ, Verstappen ECP, Ten Klooster MH and Van der Linde PCG (1995) In vitrotesting for Fusariumdisease resistance in flax. Agro Food Industry Hi Tech 6: 37-40
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Kroes, G., Sommers, E. & Lange, W. Two in vitro assays to evaluate resistance in Linum usitatissimum to Fusarium wilt disease. European Journal of Plant Pathology 104, 561–568 (1998). https://doi.org/10.1023/A:1008652611164
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DOI: https://doi.org/10.1023/A:1008652611164