Abstract
Induction of the activity of aconitate hydratase (AH) was observed in rat hepatocytes under the conditions of food deprivation. The increase in AH activity after 4 days of starvation in the studied tissues was from 0.57 to 2.05 U/g crude liver weight. The induction of aconitase was associated both with the cytoplasmic and mitochondrial AH isoforms. The activities of cytosolic and mitochondrial AH isoforms in starving animals consisted of 83 and 17% of the total activity, respectively. The cytoplasmic and mitochondrial isoforms of the enzyme with specific activities 11.1 and 6.13 U/mg protein, respectively, were obtained by a five-step purification procedure that included fractionation with ammonium sulfate, ion-exchanging chromatography on DEAE-Toyopearl and gel filtration. The purified preparations of these AH isoforms were electrophoretically homogenous. The molecular weights of these isoforms were estimated and several kinetic and regulatory properties were studied.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Lebkova, N. P. (2000) Vestnik RAMN, 9, 3-12.
Lebkova, N. P., Bondarenko, M. F., and Kolesnikova, O. E. (1980) Byul. Eksp. Biol. Med., 5, 614-617.
Popov, V. N., Igamberdiev, A. U., Schnarenberger, C., and Volvenkin, S. V. (1996) FEBS Lett., 390, 258-260.
Glusker, J. P. (1971) Enzymes, 5, 413-439.
Slaughter, C. A., Hopkinson, D. A., and Harris, H. (1975) Ann. Hum. Genet., 39, 193-202.
Prodromou, C., Artymiuk, P. J., and Guest, J. R. (1992) Eur. J. Biochem., 204, 599-609.
Scholze, H. (1983) Biochim. Biophys. Acta, 763, 133-137.
Eprintsev, A. T., Zemlyanukhin, L. A., and Aleksyuk, M. P. (1995) Biochemistry (Moscow), 80, 939-944.
Evelson, P., and Gonzalez-Flecha, B. (2000) Biochim. Biophys. Acta, 1523, 209-216.
Konstantinova, S. G., and Russanov, E. M. (1996) Comp. Biochem. Physiol. B. Biochem. Mol. Biol., 113, 125-130.
Konstantinova, S. G., Jordanova, N. G., and Russanov, E. M. (2000) Acta. Physiol. Pharmacol. Bulg., 25, 33-42.
Andersson, U., Leighton, B., Young, M. E., Blomstrand, E., and Newsholme, E. A. (1998) Biochem. Biophys. Res. Commun., 249, 512-516.
Villafranca, J. (1974) J. Biol. Chem., 249, 6149-6155.
Guarriera-Bobyleva, V., Volpi-Bobochi, M. A., and Masini, A. (1973) Eur. J. Biochem., 34, 455-458.
Zemlyanukhin, A. A., Eprintsev, A. T., Zemlyanukhin, L. A., and Igamberdiev, A. U. (1984) Fiziol. Rast., 31, 337-343.
Agrawal, P., Garg, G., and Gollakota, K. (1976) Biochem. Biophys. Res. Commun., 70, 987-996.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951) J. Biol. Chem., 193, 265-275.
Villafranca, J., and Mildvan, A. (1971) J. Biol. Chem., 246, 5791-5798.
Dixon, M., and Webb, E. (1982) Enzymes [Russian translation], Nauka, Moscow.
Eisenstein, R. S., Barton, H. A., Pettingell, W. H., Jr., and Bomford, A. B. (1997) Arch. Biochem. Biophys., 343, 81-91.
Emptage, M. H., Dreyer, J. L., Kennedy, M. C., and Beinert, H. (1993) J. Biol. Chem., 219, 11106-11111.
Beinert, H., Kennedy, M. C., and Stout, C. D. (1996) Chem. Rev., 96, 2335-2373.
Philpott, C. C., Klausner, R. D., and Rouault, T. A. (1994) Proc. Natl. Acad. Sci. USA, 9, 7321-7325.
Hochachka, P., and Somero, J. (1988) Biochemical Adaptation [Russian translation], Mir, Moscow.
Popov, V. N., Volvenkin, S. V., Eprintsev, A. T., and Igamberdiev, A. U. (1998) FEBS Lett., 440, 55-58.
Popov, V. N., Volvenkin, S. V., Eprintsev, A. T., and Igamberdiev, A. U. (2000) Izv. RAN. Ser. Biol., 6, 672-678.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Eprintsev, A.T., Semenova, E.V. & Popov, V.N. Induction of Aconitate Hydratase in Hepatocytes of Starving Rats. Biochemistry (Moscow) 67, 795–801 (2002). https://doi.org/10.1023/A:1016304909287
Issue Date:
DOI: https://doi.org/10.1023/A:1016304909287