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Generation of Transgenic Mice and Germline Transmission of a Mammalian Artificial Chromosome Introduced into Embryos by Pronuclear Microinjection

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Abstract

We have generated transgenic mice by pronuclear microinjection of a murine satellite DNA-based artificial chromosome (SATAC). As 50% of the founder progeny were SATAC-positive, this demonstrates that SATAC transmission through the germline had occurred. FISH analyses of metaphase chromosomes from mitogen-activated peripheral blood lymphocytes from both the founder and progeny revealed that the SATAC was maintained as a discrete chromosome and that it had not integrated into an endogenous chromosome. To our knowledge, this is the first report of the germline transmission of a genetically engineered mammalian artificial chromosome within transgenic animals generated through pronuclear microinjection. We have also shown that murine SATACs can be similarly introduced into bovine embryos. The use of embryo microinjection to generate transgenic mammals carrying genetically engineered chromosomes provides a novel method by which the unique advantages of chromosome-based gene delivery systems can be exploited.

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Authors and Affiliations

  1. Chromos Molecular Systems, Inc., 8081 Lougheed Highway, Burnaby, British Columbia, Canada, V5A 1W9

    Deborah O. Co, Josephine D. Leung, Carl F. Perez & Jan I. Drayer

  2. Centre for Molecular Medicine and Therapeutics, and Department of Medicine, University of British Columbia, Vancouver, British Columbia, Canada

    Anita H. Borowski & Frank R. Jirik

  3. Pharming, Transgenic Technologies, Leiden, The Netherlands

    Jos van der Kaa, Sandra Hengst, Gerard J. Platenburg & Frank R. Pieper

Authors
  1. Deborah O. Co
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  2. Anita H. Borowski
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  3. Josephine D. Leung
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  4. Jos van der Kaa
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  5. Sandra Hengst
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  6. Gerard J. Platenburg
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  7. Frank R. Pieper
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  8. Carl F. Perez
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  9. Frank R. Jirik
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  10. Jan I. Drayer
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Co, D.O., Borowski, A.H., Leung, J.D. et al. Generation of Transgenic Mice and Germline Transmission of a Mammalian Artificial Chromosome Introduced into Embryos by Pronuclear Microinjection. Chromosome Res 8, 183–191 (2000). https://doi.org/10.1023/A:1009206926548

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  • DOI: https://doi.org/10.1023/A:1009206926548

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