Hostname: page-component-848d4c4894-wg55d Total loading time: 0 Render date: 2024-05-30T23:57:15.578Z Has data issue: false hasContentIssue false
  • English
  • Français

Distribution of Aeromonas phenospecies and genospecies among strains isolated from water, foods or from human clinical samples

Published online by Cambridge University Press:  15 May 2009

M. L. Hänninen  and
A. Siitonen
M. L. Hänninen
Affiliation:
University of Veterinary Medicine, Department of Food and Environmental Hygiene, PO Box 6, 00581 Helsinki, Finland
A. Siitonen
Affiliation:
National Public Health Institute, Laboratory of Enteric Pathogens, Helsinki, Finland
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A total of 332 Aeromonas spp. originating from drinking water (n = 75), fresh water (n = 57), chicken and ground beef (107), human faecal samples in association with travelling (n = 49), human faecal samples not associated with travelling (n = 38), and six strains from human blood cultures were studied by phenotypic methods and by using analysis of ribopatterns as a molecular method for the identification of the 13 known hybridization groups (HGs). Also included were the reference strains of each HG. A. hydrophila HG 1, A. caviae HG 4 and A. veronii biotype sobria HG 8/10 were the most important genospecies identified in human faecal samples. A. hydrophila HG 2 and A. media HG 5B predominated in drinking water and A. hydrophila HG 2 and HGS, A. media HG 5A and HG 5B predominated in fresh water. In drinking water only one isolate was A. hydrophila HG 1 and two isolates were A. caviae HG 4. Clinically important Aeromonas spp. HG 1 (A. hydrophila), HG 4 (A. caviae) and HG 8/10 (A. veronii biotype sobria) were common in chicken and ground beef. In contrast to the drinking water samples, HG 5A was common in chicken and ground beef samples. Atypical, unidentified isolates were most often found in fresh water samples (12/57 strains). Although water has been suspected of being an important source of human aeromonas infections, clinically important HGs were found to be in the minority among Aeromonas spp. identified in drinking water or fresh water. The distribution of Aeromonas spp. HGs among drinking water, chicken and ground beef samples was also different, suggesting that contamination of meat or chicken may not originate from water.

Type
Research Article
Information
Epidemiology & Infection , Volume 115 , Issue 1 , August 1995 , pp. 39 - 50
Copyright
Copyright © Cambridge University Press 1995

References

1.Havelaar, AH, Schets, FM, van Silhout, A, Jansen, WH, Wieten, G, van der Kooij, D. Typing of Aeromonas strains from patients with diarrhoea and from drinking water. J Appl Bacteriol 1992; 72: 435–44.CrossRefGoogle ScholarPubMed
2.Schubert, RHW. Aeromonads and their significance as potential pathogens in water. J Appl Bacteriol Symp Suppl 1991; 70: 131S–5S.Google Scholar
3.Palumbo, SA, Bencivengo, MM, Del Corral, F, Williams, AC, Buchanan, RL. Characterization of the Aeromonas hydrophila group isolated from retail foods of animal origin. J Clin Microbiol 1989; 27: 854–9.CrossRefGoogle ScholarPubMed
4.Berrang, ME, Brackett, RE, Beuchat, LR. Growth of Aeromonas hydrophila on fresh vegetables stored under a controlled atmosphere. Appl Environ Microbiol 1989; 55: 2167–71.CrossRefGoogle Scholar
5.Fricker, CR, Tompsett, S. Aeromonas spp. in foods: a significant food poisoning ? Int J Food Microbiol 1989; 9: 17–23.CrossRefGoogle Scholar
6.Wadström, T, Ljungh, Å. Aeromonas and Plesiomonas as food- and waterborne pathogens. Int J Food Microbiol 1991; 12: 303–12.CrossRefGoogle ScholarPubMed
7.Gray, SJ, Sticker, DJ. Some observations on the faecal carriage of mesophilic Aeromonas species in cows and pigs. Epideniiol Infect 1989; 103: 523–37.CrossRefGoogle ScholarPubMed
8.Beuchat, LR. Behaviour of Aeromonas species at refrigeration temperatures. Int J Food Microbiol 1991; 13: 217–24.CrossRefGoogle ScholarPubMed
9.Hazen, TC, Fliermans, CB, Hirsch, RP, Esch, GW. Prevalence and distribution of Aeromonas hydrophila in the United States. Appl Environ Microbiol 1978; 36: 731–8.CrossRefGoogle ScholarPubMed
10.Janda, JM. Recent advances in the study of the taxonomy, pathogenicity and infections syndromes associated with the genus Aeromonas. Clin Microbiol Revs 1991; 4: 397–410.CrossRefGoogle Scholar
11.Altwegg, M. Geiss, HK. Aeromonas as human pathogen. Crit Rev Microbiol 1989; 16: 253–86.CrossRefGoogle ScholarPubMed
12.Moyer, NP, Martinetti, Lucchini G, Holcomb, LA, Hall, NH, Altwegg, M. Application of ribotyping for differentiating aeromonads isolated from clinical and environmental sources. Appl Environ Microbiol 1992; 58: 1940–4.CrossRefGoogle ScholarPubMed
13.Martinez-Murcia, AJ, Esteve, C, Garay, E, Collins, MD. Aeromonas allosaccharophila sp. nov. a new mesophilic member of the genus Aeromonas. FEMS Microbiol Lett 1992; 91: 199–206.CrossRefGoogle Scholar
14.Kämpfer, P, Altwegg, M. Numerical classification and identification of Aeromonas genospecies. J Appl Bacteriol 1992; 72: 341–51.CrossRefGoogle ScholarPubMed
15.Altwegg, M, Steigerwalt, AG, Altwegg-Bissig, R, Luthy-Hottenstein, J, Brenner, DJ. Biochemical identification of Aeromonas genospecies isolated from humans. J Clin Microbiol 1990; 28: 258–64.CrossRefGoogle ScholarPubMed
16.Abbott, SL, Cheung, WKW, Kroske-Bystrom, S, Malekzadeh, T, Janda, MJ. Identification of Aeromonas strains to the genospecies level in the clinical laboratory. J Clin Microbiol 1992; 30: 1262–6.CrossRefGoogle Scholar
17.Hänninen, ML, Salmi, S, Mattila, L, Taipalinen, R, Siitonen, A. Association of Aeromonas spp. with traveller's diarrhoea in Finland. J Med Microbiol 1995; 42: 26–31.CrossRefGoogle ScholarPubMed
18.Havelaar, AH, During, M, Versteegh, JFM. Ampicillin-dextrin agar medium for the enumeration of Aeromonas species in water by membrane filtration. J Appl Bacteriol 1987; 62: 279–87.CrossRefGoogle ScholarPubMed
19.Popoff, M. Genus III. Aeromonas. Kluyver and Van Niel 1936, 398AL. In: Krieg, NR, Holt, JG, eds. Bergey's manual of systematic bacteriology, vol 1. Baltimore: Williams and Wilkins Co. 1984: 545–8.Google Scholar
20.Carnahan, AM, Behram, S, Joseph, SW. Aerokey II: A flexible key for identifying clinical Aeromonas species. J Clin Microbiol 1991; 29: 2843–9.CrossRefGoogle Scholar
21.Martinetti, LucchiniG, Altwegg, M. rRNA restriction patterns as taxonomic tools for the genus Aeromonas. Int J Syst Bacteriol 1992; 42: 384–9.CrossRefGoogle Scholar
22.Pitcher, DG, Saunders, NA, Owen, RJ. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett Appl Microbiol 1989; 8: 151–6.CrossRefGoogle Scholar
23.Popovic, T, Bobb, CA, Olsvik, O, Kielbauch, JA. Ribotyping in molecular biology. In: Peshing, DH, Smith, TF, Tenover, FC, eds. Molecular microbiology, Principles and applications. Am Soc Microbiol, 1993: 573–83.Google Scholar
24.Altwegg, M, von Graevenitz, A, Zollinger-Iten, J. Medium and temperature dependence of decarboxylase reactions in Aeromonas spp. Curr Microbiol 1987; 15: 1–4.CrossRefGoogle Scholar
25.Carey, PE, Eley, A, Wilcox, MH. Assessment of a chemiluminescent universal probe for taxonomical and epidemiological investigations of Aeromonas sp isolates. J Clin Pathol 1994; 47: 642–6.CrossRefGoogle ScholarPubMed
26.Hänninen, ML. Phenotypic characteristics of the three hybridization groups of Aeromonas hydrophila complex. J Appl Bacteriol 1994; 76: 455–62.CrossRefGoogle Scholar
27.Hänninen, ML, Salmi, S, Siitonen, A. Maximum growth temperature ranges of Aeromonasspp. isolated from clinical or environmental sources. Microbial Ecol 1995: In press.CrossRefGoogle ScholarPubMed
28.Kuijper, EJ, Steigerwalt, AG, Schoenmakers, BSCIM, Peeters, MF, Zanen, HC, Brenner, DJ. Phenotypic characterization and DNA relatedness in human fecal isolates of Aeromonas spp. J Clin Microbiol 1989; 27: 132–9.CrossRefGoogle ScholarPubMed
29.Moyer, NB. Clinical significance of Aeromonas species isolated from patients with diarrhea. J Clin Microbiol 1987; 25: 2044–8.CrossRefGoogle ScholarPubMed
30.Kirov, SM. The public health significance of Aeromonas spp. in foods. Int J Food Microbiol 1993; 20: 179–98.CrossRefGoogle ScholarPubMed
31.Carnahan, AM, Chakrabotry, T, Fanning, GR et al. , Aeromonas trota sp. nov. an ampicillin-susceptible species isolated from clinical specimens. J Clin Microbiol 1991; 29: 1206–10.CrossRefGoogle ScholarPubMed
32.Allen, DA, Austin, AB, Colwell, RR. Aeromonas media, a new species isolated from river water. Int J Syst Bacteriol 1983; 33: 599–603.CrossRefGoogle Scholar
33.Schubert, RW, Hegazi, M. Aeromonas eucrenophila species nova Aeromonas caviae a later and illegitimate synonym of Aeromonas punctata. Zentralbl Bacteriol Microbiol Hyg A 1988: 268: 34–9.Google ScholarPubMed
34.Burke, V, Robinson, J, Gracey, M, Peterson, D, Meyer, N, Haley, V. Isolation of Aeromonas hydrophila from a metropolitan water supply: seasonal correlation with clinical isolates. Appl Environ Microbiol 1984; 48: 361–6.CrossRefGoogle ScholarPubMed
35.Namdari, H, Bottone, EJ. Aeromonas species: Pathogens of aquatic inhabitants with a human host range. Clin Microbiol Newslett 1991; 13: 113–6.CrossRefGoogle Scholar