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Use of body fluid of adult female Ascaris suum as an antigen in the enzyme-linked immunosorbent assay (ELISA) for diagnosis of swine ascariosis

Published online by Cambridge University Press:  05 June 2009

S. Yoshihara ,
T. Oya ,
T. Furuya  and
N. Goto
S. Yoshihara
Affiliation:
Kyushu Branch Laboratory, National Institute of Animal Health, Chuzan-cho, Kagoshima-shi, Kagoshima 891-01, Japan
T. Oya
Affiliation:
Kyushu Branch Laboratory, National Institute of Animal Health, Chuzan-cho, Kagoshima-shi, Kagoshima 891-01, Japan
T. Furuya
Affiliation:
Agricultural Technical Center, Pfizer Pharmaceuticals Inc., Kannami-cho, Shizuoka 419-01, Japan
N. Goto
Affiliation:
Department of Safety Research on Biologics, National Institute of Health, Gakuen, Musashimurayama-shi, Tokyo 208, Japan
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Abstract

The Enzyme-linked immunosorbent assay (ELISA) was used to detect Ascaris suum antibodies in swine sera with adult body fluid (ABF) as an antigen. The assay was standardized with respect to the antigen concentration and serum and conjugate dilutions. Cross reaction was found between the antigen and the sera from the swine infected with Metastrongylus apri. The ELISA was more sensitive than the complement fixation test. Five protein peaks were obtained from ABF by gel filtration on Sephacryle S-300. Fraction I was the most specific. A 105 kDa protein in the fraction reacted with swine lgG in the serum of infected animals in Western blot analysis.

Keywords

Ascaris suumadult body fluidELISAWestern blot analysisNematoda
Type
Research Papers
Information
Journal of Helminthology , Volume 67 , Issue 4 , December 1993 , pp. 279 - 286
Copyright
Copyright © Cambridge University Press 1993

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