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Assessment of different selective agar media for enumeration and isolation of Listeria from dairy products

Published online by Cambridge University Press:  01 June 2009

Lucas Dominguez ,
José Francisco Fernández ,
Victor Briones ,
José Luis Blanco  and
Guillermo Suárez
Lucas Dominguez
Affiliation:
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
José Francisco Fernández
Affiliation:
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
Victor Briones
Affiliation:
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
José Luis Blanco
Affiliation:
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
Guillermo Suárez
Affiliation:
Departamento de Patología Animal I (Sanidad Animal), Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain
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Summary

Different selective agar media were compared for the recovery and isolation of five species of Listeria from raw milk and cheese. The selective media examined were Beerens medium, MacBride medium and that described by Dominguez et al. (1984) with 6 mg/1 acriflavine, listeria selective agar medium (LSAM), and LSAM with 12 mg/1 acriflavine (LSAM × 2A); a non-selective yeast glucose Lemco agar was included for comparison. When the difference between listeria and the natural microflora of raw milk and cheese was 102 cfu/ml, listeria could be isolated by direct plating on all media tested. When it was lower than 103–104 cfu/ml, listeria were isolated by direct plating only on LSAM and LSAM × 2A. When the difference was greater than 104 cfu/ml, a previous enrichment was necessary to isolate them. LSAM and LSAM × 2A media performed better than the other media tested for isolating listeria by direct plating and improved their isolation from dairy products. This superior performance was evaluated by the ability of these media to support colony formation of different species of Listeria tested, the easy recognition of these colonies from those formed by other microorganisms and by their capacity to inhibit the natural microflora of these foods.

Type
Original articles
Information
Journal of Dairy Research , Volume 55 , Issue 4 , November 1988 , pp. 579 - 583
Copyright
Copyright © Proprietors of Journal of Dairy Research 1988

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References

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