Characteristics of some single-step mutants to chloramphenicol resistance in Escherichia coli K12 and their interactions with R-factor genes

E. C. R. Reeve

doi:10.1017/S0016672300009708

Extract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Six one-step Chloramphenicol (Cm)-resistant mutants of Escherichia coli K12 were graded for resistance to Cm, Tetracycline (Tc) and Puromyein (Pm) by streaking on minimal agar plates containing antibiotic. They fell into at least three distinct groups on the basis of their resistance patterns. One mutant showed increased sensitivity to Pm. Most of the mutants expressed their effect on resistance to Cm and Tc in the presence of R-factors carrying resistance genes for these antibiotics, but one mutant with a relatively high level of resistance to Cm had its resistance effect completely masked in the presence of R-mediated resistance. Similar cases were found among mutants selected for Cm-resistance in another strain of K12.

References

REFERENCES

Adams, M. H. (1959). Bacteriophages. New York: Interscience Publishers.Google Scholar

Meytstell, E. & Datta, N. (1966). The relation of resistance transfer factors to the F-factor (sex-factor) of Escherichia coli K12. Genet. Res. 7, 134–140.Google Scholar

Okamoto, S. & Mizuno, D. (1964). Mechanism of chloramphenicol and tetracycline resistance in Escherichia coli. J. gen. Microbiol. 35, 125–133.Google Scholar

Okamoto, S. & Suzuki, Y. (1965). CMoramphenicol-.Dihydrostreptomycin-, and Kanamycin. inactivating enzymes from multiple drug-resistant Escherichia coli carrying Episome ‘R’. Nature, Lond. 208, 1301–1303.CrossRef Google Scholar PubMed

Reeve, E. C. R. & Bishop, J. O. (1965 a). Multi-step resistance to chloramphenicol in RC-stringent Escherichia coli K12—its effect on the induction of RNA synthesis by antibiotics under amino-acid starvation. Genet. Res. 6, 304–309.Google Scholar

Reeve, E. C. R. & Bishop, J. O. (1965 b). Variations in resistance to three antibiotics among some single-step mutants to chloramphenicol resistance in a strain of Escherichia coli K12. Genet. Res. 6, 310–315.Google Scholar

Watanabe, T. (1963). Infective heredity of multiple drug resistance in bacteria. Bact. Rev. 27, 87–114.Google Scholar

Crossref Citations

This article has been cited by the following publications. This list is generated based on data provided by Crossref.

Wilkie, D. Saunders, G. and Linnane, Anthony W. 1967. Inhibition of respiratory enzyme synthesis in yeast by chloramphenicol: Relationship between chloramphenicol tolerance and resistance to other antibacterial antibiotics. Genetical Research, Vol. 10, Issue. 2, p. 199.

Reeve, E. C. R. 1968. Genetic analysis of some mutations causing resistance to tetracycline inEscherichia coliK12. Genetical Research, Vol. 11, Issue. 3, p. 303.

Nordström, Kurt Eriksson-Grennberg, Kerstin G. and Boman, Hans G. 1968. Resistance ofEscherichia colito penicillins: III. AmpB, a locus affecting episomally and chromosomally mediated resistance to ampicillin and chloramphenicol. Genetical Research, Vol. 12, Issue. 2, p. 157.

Reeve, E. C. R. and Suttie, D. R. 1968. Chromosomal location of a mutation causing chloramphenicol resistance inEscherichia coliK 12. Genetical Research, Vol. 11, Issue. 1, p. 97.

Roberts, Lilian M. and Reeve, E. C. R. 1970. Two mutations giving low-level streptomycin resistance inEscherichia coliK12. Genetical Research, Vol. 16, Issue. 3, p. 359.

Shaw, William V. 1971. Vol. 9, Issue. , p. 131.

CrossRef

Robertson, J. M. and Reeve, E. C. R. 1972. Analysis of the resistance mediated by several R-factors to Tetracycline and Minocycline. Genetical Research, Vol. 20, Issue. 2, p. 239.

Nagai, Yutaka and Mitsuhashi, Susumu 1972. New Type of R Factors Incapable of Inactivating Chloramphenicol. Journal of Bacteriology, Vol. 109, Issue. 1, p. 1.

Reeve, E. C. R. and Robertson, J. M. 1975. The characteristics of eleven mutants of R-factor R57 constitutive for tetracycline resistance, selected and tested inEscherichia coliK12. Genetical Research, Vol. 25, Issue. 3, p. 297.

Foulds, J 1976. TolF locus in Escherichia coli: chromosomal location and relationship to loci cmlB and tolD. Journal of Bacteriology, Vol. 128, Issue. 2, p. 604.

Chai, T J and Foulds, J 1977. Escherichia coli K-12 tolF mutants: alterations in protein composition of the outer membrane. Journal of Bacteriology, Vol. 130, Issue. 2, p. 781.

Baughman, G A and Fahnestock, S R 1979. Chloramphenicol resistance mutation in Escherichia coli which maps in the major ribosomal protein gene cluster. Journal of Bacteriology, Vol. 137, Issue. 3, p. 1315.

Chai, T Wu, V and Foulds, J 1982. Colicin A receptor: role of two Escherichia coli outer membrane proteins (OmpF protein and btuB gene product) and lipopolysaccharide. Journal of Bacteriology, Vol. 151, Issue. 2, p. 983.

Shaw, William V. 1983. Chloramphenicol Acetyltransferase: Enzymology and Molecular Biology. Critical Reviews in Biochemistry, Vol. 14, Issue. 1, p. 1.

Barcak, G J and Burchard, R P 1985. Induction of chloramphenicol and tetracycline resistance in Flexibacter sp. strain FS-1. Journal of Bacteriology, Vol. 161, Issue. 2, p. 810.

Burns, J L Mendelman, P M Levy, J Stull, T L and Smith, A L 1985. A permeability barrier as a mechanism of chloramphenicol resistance in Haemophilus influenzae. Antimicrobial Agents and Chemotherapy, Vol. 27, Issue. 1, p. 46.

Chopra, I. 1985. The Tetracyclines. Vol. 78, Issue. , p. 317.

Burns, J L and Smith, A L 1987. Chloramphenicol accumulation by Haemophilus influenzae. Antimicrobial Agents and Chemotherapy, Vol. 31, Issue. 5, p. 686.

Bohn, Chantal and Bouloc, Philippe 1998. The Escherichia coli cmlA Gene Encodes the Multidrug Efflux Pump Cmr/MdfA and Is Responsible for Isopropyl-β- d -Thiogalactopyranoside Exclusion and Spectinomycin Sensitivity . Journal of Bacteriology, Vol. 180, Issue. 22, p. 6072.

Article contents

Characteristics of some single-step mutants to chloramphenicol resistance in Escherichia coli K12 and their interactions with R-factor genes

Extract

References

REFERENCES

Save article to Kindle

Save article to Dropbox

Save article to Google Drive

Reply to: Submit a response

Your details

You have entered the maximum number of contributors

Conflicting interests