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Laboratory-based study of novel antimicrobial cold spray coatings to combat surface microbial contamination

Published online by Cambridge University Press:  19 August 2020

M.D.I. Lucas ,
I. Botef ,
R. G. Reid Open the ORCID record for R. G. Reid [Opens in a new window]  and
S. F. van Vuuren Open the ORCID record for S. F. van Vuuren [Opens in a new window]
M.D.I. Lucas
Affiliation:
School of Mechanical, Industrial and Aeronautical Engineering, Faculty of Engineering and the Built Environment, University of the Witwatersrand, Braamfontein, Johannesburg, South Africa
I. Botef
Affiliation:
School of Mechanical, Industrial and Aeronautical Engineering, Faculty of Engineering and the Built Environment, University of the Witwatersrand, Braamfontein, Johannesburg, South Africa
R. G. Reid
Affiliation:
School of Mechanical, Industrial and Aeronautical Engineering, Faculty of Engineering and the Built Environment, University of the Witwatersrand, Braamfontein, Johannesburg, South Africa
S. F. van Vuuren*
Affiliation:
Department of Pharmacy and Pharmacology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, Johannesburg, South Africa
*
Author for correspondence: Sandy van Vuuren, Email: sandy.vanvuuren@wits.ac.za
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Abstract

Objective:

To investigate the touch-contact antimicrobial efficacy of novel cold spray surface coatings composed of copper and silver metals, regard to their rate of microbial elimination.

Design:

Antimicrobial time-kill assay.

Setting:

Laboratory-based study.

Methods:

An adapted time-kill assay was conducted to characterize the antimicrobial efficacy of the developed coatings. A simulated touch-contact pathogenic exposure to Gram-positive Staphylococcus aureus (ATCC 25923), Gram-negative Pseudomonas aeruginosa (ATCC 27853), and the yeast Candida albicans (ATCC 10231), as well as corresponding resistant strains of gentamicin-methicillin–resistant S. aureus (ATCC 33592), azlocillin-carbenicillin–resistant P. aeruginosa (DSM 46316), and a fluconazole-resistant C. albicans strain was undertaken. Linear regression modeling was used to deduce microbial reduction rates.

Results:

A >7 log reduction in microbial colony forming units was achieved within minutes on surfaces with cold spray coatings compared to a single log bacterial reduction on copper metal sheets within a 3 hour contact period. Copper-coated 3-dimensional (3D) printed acrylonitrile butadiene styrene (ABS) achieved complete microbial elimination against all tested pathogens within a 15 minute exposure period. Similarly, a copper-on-copper coating achieved microbial elimination within 10 minutes and within 5 minutes with the addition of silver powder as a 5 wt% coating constituent.

Conclusions:

In response to the global need for alternative solutions for infection control and prevention, these effective antimicrobial surface coatings were proposed. A longitudinal study is the next step toward technology integration.

Type
Original Article
Information
Infection Control & Hospital Epidemiology , Volume 41 , Issue 12 , December 2020 , pp. 1378 - 1383
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Copyright
© 2020 by The Society for Healthcare Epidemiology of America. All rights reserved.

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Footnotes

a

Deceased.

References

Schettler, T. Antimicrobials in hospital furnishings: do they help reduce healthcare-associated infections? US & Canada: Healthcare Without Harm. Safer Chemicals Program, 2016. Healthcare Without Harm website. https://noharm-uscanada.org/documents/antimicrobials-hospital-furnishings-do-they-help-reduce-healthcare-associated-infections. Published 2016. Accessed November 2019.Google Scholar
Ventola, CL. The antibiotic resistance crisis: part 1: causes and threats. Pharm Therapeut 2015;40:277–283.Google ScholarPubMed
Kandel, CE, Simor, AE, Redelmeier, DA. Elevator buttons as unrecognized sources of bacterial colonization in hospitals. Open Med 2014;8:e81–e86.Google ScholarPubMed
Messina, G, Ceriale, E, Lenzi, D, Burgassi, S, Azzolini, E, Manzi, P. Environmental contaminants in hospital settings and progress in disinfecting techniques. Biomed Res Int 2013;429780. doi: 10.1155/2013/429780.Google ScholarPubMed
WHO guidelines on hand hygiene in health care: a summary. World Health Organization website. https://www.who.int/gpsc/5may/tools/9789241597906/en/. Published 2009. Accessed November 2018.Google Scholar
Hand hygiene in healthcare settings. Centers for Disease Control and Prevention website. https://www.cdc.gov/handhygiene/index.html. Updated April 2019. Accessed November 2019.Google Scholar
Villapún, VM, Dover, LG, Cross, A, González, S. Antibacterial metallic touch surfaces. Materials 2016;9:736.CrossRefGoogle ScholarPubMed
Mann, EE, Mettetal, MR, May, RM, et al. Surface micropattern resists bacterial contamination transferred by healthcare practitioners. J Microbiol Exp 2014;1. doi: 10.15406/jmen.2014.01.00032.Google Scholar
Garza-Cervantes, JA, Chávez-Reyes, A, Castillo, EC, et al. Synergistic antimicrobial effects of silver/transition-metal combinatorial treatments. Sci Rep 2017;7. doi: 10.1038/s41598-017-01017-7.Google ScholarPubMed
Grass, G, Rensing, C, Solioz, M. Metallic copper as an antimicrobial surface. Appl Environ Microbiol 2011;77:1541–1547.CrossRefGoogle ScholarPubMed
Casey, AL, Adams, D, Karpanen, TJ, et al. Role of copper in reducing hospital environment contamination. J Hosp Infect 2010;74:72–77.CrossRefGoogle ScholarPubMed
Marais, F, Mehtar, S, Chalkley, L. Antimicrobial efficacy of copper touch surfaces in reducing environmental bioburden in a South African community healthcare facility. J Hosp Infect 2010;74:80–95.Google Scholar
Hans, M, Támara, JC, Mathews, S, et al. Laser cladding of stainless steel with a copper-silver alloy to generate surfaces of high antimicrobial activity. Appl Surf Sci 2014;320:195–199.Google Scholar
Sharifahmadian, O, Salimijazi, HR, Fathi, MH, Mostaghimi, J, Pershin, L. Study of the antibacterial behavior of wire arc sprayed copper coatings. J Therm Spray Technol 2013;22:371–379.CrossRefGoogle Scholar
Champagne, VK, Helfritch, DJ. A Demonstration of the antimicrobial effectiveness of various copper surfaces. J Biol Eng 2013;7. doi: 10.1186/1754-1611-7-8.CrossRefGoogle ScholarPubMed
Updated draft protocol for the evaluation of bactericidal activity of hard, non-porous copper containing surface products. United States Environmental Protection Agency website. https://www.epa.gov/pesticide-registration/updated-draft-protocol-evaluation-bactericidal-activity-hard-non-porous. Published 2016. Accessed May 2019.Google Scholar
Lucas, MDI, Botef, I, van Vuuren, S, inventors; The University of the Witwatersrand, Johannesburg, assignee. Method of Applying an Antimicrobial Surface Coating to a Substrate, 2019, International publication number WO 2019/064216 AI. 2019 April 4.Google Scholar
Santajit, S, Indrawattana, N. Mechanisms of antimicrobial resistance in ESKAPE pathogens. BioMed Res Int 2016. doi: 10.1155/2016/2475067.CrossRefGoogle ScholarPubMed
Montgomery, DC, Runger, GC. Applied Statistics and Probability for Engineers, 6th Edition. Singapore: John Wiley & Son; 2014.Google Scholar
Lucas, MDI. Antimicrobial surface coatings via cold spray and 3D printing technologies [PhD thesis]. University of the Witwatersrand, Johannesburg; submitted 2020.Google Scholar
Botef, I. Manufacturing—Design and Processes. Sandton, South Africa: Picsie Press; 2013.Google Scholar
Shrestha, R, Joshi, DR, Gopali, J, Piya, S. Oligodynamic action of silver, copper and brass on enteric bacteria isolated from water of Kathmandu valley. Nepal J Sci Tech 2009;10:189–193.CrossRefGoogle Scholar
Lemire, JA, Harrison, JJ, Turner, RJ. Antimicrobial activity of metals: mechanisms, molecular targets and applications. Nat Rev Microbiol 2013;11:371–384.Google ScholarPubMed