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Identification and expression analysis of Dazl homologue in Cynops cyanurus

Published online by Cambridge University Press:  28 July 2021

Yinjiao Zhao ,
Ya Du ,
Qinglan Ge ,
Fang Yan  and
Shu Wei Open the ORCID record for Shu Wei [Opens in a new window]
Yinjiao Zhao
Affiliation:
State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, Yunnan, China Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, Yunnan, China
Ya Du
Affiliation:
Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, Yunnan, China
Qinglan Ge
Affiliation:
Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming, Yunnan, China
Fang Yan*
Affiliation:
State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, Yunnan, China
Shu Wei*
Affiliation:
State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, Yunnan, China
*
Authors for correspondence: Fang Yan and Shu Wei. State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, Yunnan, China. E-mail: shuwei@ynu.edu.cn; fangyan@ynu.edu.cn
Authors for correspondence: Fang Yan and Shu Wei. State Key Laboratory of Conservation and Utilization of Bio-Resources in Yunnan and Center for Life Science, School of Life Sciences, Yunnan University, Kunming, Yunnan, China. E-mail: shuwei@ynu.edu.cn; fangyan@ynu.edu.cn
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Summary

The Dazl (deleted in azoospermia-like) gene encodes an RNA-binding protein containing an RNA recognition motif (RRM) and a DAZ motif. Dazl is essential for gametogenesis in vertebrates. In this study, we report the cloning of Dazl cDNA from Cynops cyanurus. Ccdazl mRNA showed a germline-specific expression pattern as expected. Ccdazl expression gradually decreased during oogenesis, suggesting that it may be involved in oocyte development. Phylogenetic analysis revealed that the Ccdazl protein shares conserved motifs/domains with Dazl proteins from other species. Cloning of Ccdazl provides a new tool to carry out comparative studies of germ cell development in amphibians.

Keywords

Cynops cyanurusDAZ familyDazlGerm cellNewt
Type
Research Article
Information
Zygote , Volume 30 , Issue 2 , April 2022 , pp. 221 - 226
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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