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Structural analysis of fertilization in the fish Brycon orbignyanus

Published online by Cambridge University Press:  01 May 2009

Luciana Nakaghi Ganeco ,
Irene Bastos Franceschini-Vicentini  and
Laura Satiko Okada Nakaghi
Luciana Nakaghi Ganeco*
Affiliation:
Departamento de Morfologia e Fisiologia Animal, UNESP–São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP, Brazil. Aquaculture Center (CAUNESP)–Jaboticabal-SP, Brazil.
Irene Bastos Franceschini-Vicentini
Affiliation:
Aquaculture Center (CAUNESP)–Jaboticabal-SP, Brazil. Depto. Ciências Biológicas, Faculdade de Ciências–UNESP, Bauru-SP, Brazil.
Laura Satiko Okada Nakaghi
Affiliation:
Aquaculture Center (CAUNESP)–Jaboticabal-SP, Brazil. Depto. Morfologia e Fisiologia Animal, Faculdade de Ciências Agrárias e Veterinárias–UNESP, Jaboticabal-SP, Brazil.
*
All correspondence to: L. Nakaghi Ganeco. Departamento de Morfologia e Fisiologia Animal, UNESP–São Paulo State University, Via de Acesso Prof. Paulo Donato Castellane, s/n, CEP 14884-900, Jaboticabal, SP, Brazil. Tel:/Fax: +55 16 3209 2654. e-mail: lnganeco@yahoo.com.br
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Summary

In the present work, we analyzed the structure of oocytes and fertilized eggs of the piracanjuba fish (Brycon orbignyanus) under light and scanning electron microscopy. After inducing spawning, samples were collected at the moment of oocyte extrusion, when oocytes and semen were mixed (time 0), as well as at 10, 20 and 30 s after mixing, every minute up to 10 min, and then at 15 and 20 min. The oocytes are spherical, translucent and greenish with a mean diameter of 1.3 ± 0.11 mm. During the extrusion, cytoplasmic movement was observed in eggs towards the micropyle, characterizing the animal pole. At the moment of fertilization, the cortical cytoplasm showed a higher concentration of cortical alveoli at the animal pole than at the vegetal pole. The cortical alveoli breakdown promoted the elevation of the chorion with a consequent increase in egg diameter (1.95 ± 0.08 mm). The penetration of the spermatozoon promotes the formation of a fertilization cone of spherical external structure, which obstructs the opening of the micropyle. This structure acts as a main mechanism to avoid polyspermy, intercepting the access of supernumerary spermatozoa. Such studies about the reproductive biology of fish are important to species survival and conservation programmes.

Keywords

EggFertilizationFishMicropyleMicroscopy
Type
Research Article
Information
Zygote , Volume 17 , Issue 2 , May 2009 , pp. 93 - 99
Copyright
Copyright © Cambridge University Press 2008

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