Taxonomic position and intraspecific variability of the nodule forming Penicillium nodositatum inferred from RFLP analysis of the ribosomal intergenic spacer and Random Amplified Polymorphic DNA

doi:10.1017/S0953756296002857

Mycological Research

Volume 101, Issue 4, April 1997, Pages 465-472

https://doi.org/10.1017/S0953756296002857 Get rights and content

Penicillium nodositatum induces the formation of nodules on the roots of Alnus. The analysis of RFLPs in the PCR-amplified ITS1-5·8S-ITS2 ribosomal sequence demonstrates that all the nodule-inducing strains isolated belong to a homogeneous taxon, P. nodositatum, distinct from other morphologically close species of Penicillium. A gene tree inferred from the ITS1-5·8S-ITS2 PCR/RFLP data shows that the P. nodositatum sequence clusters with those of Penicillium species belonging to subgenus Biverticillium. RAPDs generated using four 10-mer primers, allowed us to identify four distinct groups of strains in a sample of 28 strains of P. nodositatum collected in 10 different geographic sites. Only one group was found in each sampled site. No link could be established between RAPD-based groups and the geographic origin of the strains. However, our results suggest a possible link between these groups and Alnus species and/or habitats associated with each of the four French Alnus species.

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Taxonomic position and intraspecific variability of the nodule forming Penicillium nodositatum inferred from RFLP analysis of the ribosomal intergenic spacer and Random Amplified Polymorphic DNA

Mycological Research

Mycological Research

Mycological Research

Taxonomic implication of hybridization of Penicillium protoplasts.

Differentiation of Fusarium oxysporum f. sp. vasinfectum races on cotton by random amplified polymorphic DNA (RAPD) analysis

Molecular Plant Pathology

Is Penicillium monophyletic. An evaluation of phylogeny in the family Trichocomaceae from 18S, 5.8S and ITS ribosomal DNA sequence data

Mycologia

Root-nodules formation by Penicillium sp. on Alnus glutinosa and Alnus incana

Plant and Soil

Detection of molecular variation in the insect pathogenic fungus Metarhizium using RAPD–PCR

FEMS Microbiology Letters

Identification of the ectomycorrhizal basidiomycete Tylospora fibrillosa Donk by RFLP analysis of the PCR-amplified ITS and IGS regions of ribosomal DNA

Mycological Research

Intraspecific variability of isozymes in Penicillium nodositatum Valla, a fungus inducing ‘myconodules’ on the root-system of Alnus sp

Plant and Soil

Construction of phylogenetic trees

Science

Applied and Environmental Microbiology

ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts

Molecular Ecology

Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA

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Proceedings of the National Academy of Sciences