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Clonal dissemination of Vibrio parahaemolyticus displaying similar DNA fingerprint but belonging to two different serovars (O3[ratio ]K6 and O4[ratio ]K68) in Thailand and India

Published online by Cambridge University Press:  03 November 2000

N. R. CHOWDHURY ,
S. CHAKRABORTY ,
B. EAMPOKALAP ,
W. CHAICUMPA ,
M. CHONGSA-NGUAN ,
P. MOOLASART ,
R. MITRA ,
T. RAMAMURTHY ,
S. K. BHATTACHARYA  and
M. NISHIBUCHI
...Show all authors
N. R. CHOWDHURY
Affiliation:
National Institute of Cholera and Enteric Diseases, Calcutta, India
S. CHAKRABORTY
Affiliation:
National Institute of Cholera and Enteric Diseases, Calcutta, India
B. EAMPOKALAP
Affiliation:
Bamrasnaradura Infectious Diseases Hospital, Nonthaburi Province, Thailand
W. CHAICUMPA
Affiliation:
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
M. CHONGSA-NGUAN
Affiliation:
Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand
P. MOOLASART
Affiliation:
Bamrasnaradura Infectious Diseases Hospital, Nonthaburi Province, Thailand
R. MITRA
Affiliation:
National Institute of Cholera and Enteric Diseases, Calcutta, India
T. RAMAMURTHY
Affiliation:
National Institute of Cholera and Enteric Diseases, Calcutta, India
S. K. BHATTACHARYA
Affiliation:
National Institute of Cholera and Enteric Diseases, Calcutta, India
M. NISHIBUCHI
Affiliation:
Center for Southeast Asian Studies, Kyoto University, Kyoto, Japan
Y. TAKEDA
Affiliation:
National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan
G. BALAKRISH NAIR
Affiliation:
National Institute of Cholera and Enteric Diseases, Calcutta, India
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Abstract

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Active surveillance of Vibrio parahaemolyticus infection among hospitalized patients in Calcutta, India, showed the appearance of the O4[ratio ]K68 serovar for the first time in March 1998 alongside the continued predominant incidence of the O3[ratio ]K6 serovar. Strains belonging to both these serovars have been reported to possess pandemic potential. The genomes of O3[ratio ]K6 and O4[ratio ]K68 strains and for comparison, non-O3[ratio ]K6 and non-O4[ratio ]K68 strains isolated from two different countries, India and Thailand, were examined by different molecular techniques to determine their relatedness. The O3[ratio ]K6 and O4[ratio ]K68 strains from Calcutta and Bangkok carried the tdh gene but not the trh gene. Characterization of representative strains of these two serovars by ribotyping and by arbitrarily primed-polymerase chain reaction (AP-PCR) showed that the isolates had identical ribotype and DNA fingerprint. Pulsed-field gel electrophoresis (PFGE) performed with the same set of strains yielded nearly similar restriction fragment length polymorphism (RFLP) patterns for the O3[ratio ]K6 and O4[ratio ]K68 isolates from Calcutta and Thailand. Phylogenetic analysis of the NotI RFLP showed that the O3[ratio ]K6 and O4[ratio ]K68 strains formed a cluster with 78–91% similarity thus indicating close genetic relationship between the two different serovars isolated during the same time-frame but from widely separated geographical regions. The non-O3[ratio ]K6 and non-O4[ratio ]K68, in contrast, showed different ribotype, AP-PCR and PFGE patterns.

Type
Research Article
Information
Epidemiology & Infection , Volume 125 , Issue 1 , August 2000 , pp. 17 - 25
Copyright
© 2000 Cambridge University Press