Hostname: page-component-76fb5796d-45l2p Total loading time: 0 Render date: 2024-04-25T16:04:39.826Z Has data issue: false hasContentIssue false
  • English
  • Français

A competitive ELISA for the detection of group-specific antibodies to African horse sickness virus

Published online by Cambridge University Press:  15 May 2009

C. Hamblin ,
S. D. Graham ,
E. C. Anderson  and
J. R. Crowther
C. Hamblin
Affiliation:
Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey, GU24 ONF
S. D. Graham
Affiliation:
Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey, GU24 ONF
E. C. Anderson
Affiliation:
Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey, GU24 ONF
J. R. Crowther
Affiliation:
Department of Virus Diagnosis, AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey, GU24 ONF
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A competition enzyme-linked immunosorbent assay (ELISA) has been developed for the rapid identification and quantification of antibodies against African horse sickness (AHS) in sera from solipeds. The data showed the ELISA to be sensitive, specific and reliable.

More than 1600 sera from 37 different countries were examined and results compared with those obtained by agar gel immuno-diffusion (AGID) tests. In no case did any of 775 sera from countries where AHS has never been reported and where AHS vaccines are not used, record an ELISA titre greater than 4. A titre equal to or greater than 8 was considered positive. Using this criterion, 96·3% of sera tested in both assays were in agreement. Doubtful results by AGID (1·7%) were clearly defined in terms of positivity and negativity by ELISA.

This ELISA is suited for the rapid laboratory confirmation of AHS and should be considered as a replacement for the traditional AGID test.

Type
Research Article
Information
Epidemiology & Infection , Volume 104 , Issue 2 , April 1990 , pp. 303 - 312
Copyright
Copyright © Cambridge University Press 1990

References

REFERENCES

Chalmers, AW, African horse sickness. Equine Vet J 1968; 1: 1–4.Google Scholar
Hess, WR.African horse sickness. In The arboviruses: Epidemiology and ecology, ed TP, Monath, Vol. II. Boca Raton, Florida:CRC Press Inc. 1988: 1–18.Google Scholar
Williams, R. A single dilution enzyme-linked immunosorbent assay for the quantitative detection of antibodies to African horse sickness virus. Onderstepoort J Vet Res 1987; 54: 67–70.Google Scholar
Anderson, J. Investigations on Bluetongue virus using monoclonal antibodies (PhD Thesis). Council for National Academic Awards,London, 1986; 292 pp.Google Scholar
Afshar, A, Thomas, FC, Wright, PF, Shapiro, JL, Shettigara, PT, Anderson, J. Comparison of competitive and indirect enzyme-linked immunosorbent assays for detection of Bluetongue virus antibodies in serum and whole blood. J Clin Microbiol 1987; 25: 1705–10.Google Scholar
Voller, A, Bidwell, DE, Bartlett, A. The enzyme linked immunosorbent assay (ELISA): a guide with abstracts of microplate applications.London:Zoological Society of London, 1979; 128 pp.Google Scholar
Eisa, M, McGrane, J, Taylor, WP, Ballouh, LA. Survey of precipitating antibodies to bluetongue virus in domestic animals in Sudan. Bull Anim Health Prod Afr 1983; 31: 95–9.Google Scholar