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Further observations on the serological response to experimental Salmonella typhimurium in chickens measured by ELISA

Published online by Cambridge University Press:  15 May 2009

P. A. Barrow
P. A. Barrow
Affiliation:
AFRC-Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA
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Summary

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An indirect ELISA developed for the serological detection of Salmonella typhimurium in chickens using lipopolysaccharide as detecting antigen has been evaluated further in experimental infections. Following oral infection of 24-week-old laying hens with an invasive strain of S. typhimurium, high titres of specific circulating IgG were induced which were maintained for 20 weeks. Similar IgG titres were found in egg yolk. When 4-day-old chickens were infected high antibody titres persisted for 45 weeks. Chickens inoculated orally or intramuscularly with different numbers of S. typhimurium organisms showed graded serum IgG responses to LPS. The IgG titres in experimentally infected in-bred lines of chickens which showed greater genetic resistance to salmonella infection were significantly lower than those found in more susceptible lines. Oral and intramuscular infection with 18 different types of enterobacteria, including avian pathogenic E. coli, Citrobacter spp., Klebsiella spp., Proteus spp. and citrobacter-like organisms possessing some salmonella LPS (none possessed the 0–4 antigen) and flagella antigens, did not induce S. typhimurium LPS-specific IgG responses. Chickens infected orally with rough or non-flagellate mutants of S. typhimurium did not induce high titres of LPS or flagella-specific IgG respectively. Sera obtained from S. typhimurium-infected chickens showed much higher titres against S. typhimurium LPS than with those antigens from other serotypes, including S. enteritidis.

Type
Research Article
Information
Epidemiology & Infection , Volume 108 , Issue 2 , April 1992 , pp. 231 - 242
Copyright
Copyright © Cambridge University Press 1992

References

REFERENCES

1.Nagaraja, KV, Emery, DA, Sherlock, LF, Newman, JS, Pomeroy, BS. Detection of Salmonella arizonae in turkey flocks by ELISA.Proceedings of the 27th Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians1984: 185–204.Google Scholar
2.Nagaraja, KV, Ausherman, LT, Emery, DA, Pomeroy, BS. Update on enzyme linked immunosorbent assay for its field application in the detection of Salmonella arizonae infection in breeder flocks of turkeys.Proceedings of the 29th Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians1986; 347–56.Google Scholar
3.Barrow, PA, Hassan, JO, Mockett, APA, McLeod, S. Detection of Salmonella infection by ELISA. Vet Rec 1989; 125: 586.Google ScholarPubMed
4.Dadrast, H, Hesketh, R, Taylor, DJ. Egg yolk antibody detection in identification of Salmonela infected poultry. Vet Rec 1990; 126: 219.Google Scholar
5.Hassan, JO, Barrow, PA, Mockett, APA, McLeod, S. Antibody response to experimental Salmonella typhimurium infection in chickens measured by ELISA. Vet Rec 1990; 126: 519–22.Google ScholarPubMed
6.Cooper, GL, Nicholas, RAJ, Bracewell, CD. Serological and bacteriological investigation of chickens from flocks naturally infected with Salmonella enteritidis. Vet Rec 1989; 125: 567–72.Google ScholarPubMed
7.Chart, H, Rowe, B, Baskerville, A, Humphrey, TJ. Serological response of chickens to Salmonella enteritidis infection. Epidmiol Infect 1990; 104: 63–71.CrossRefGoogle ScholarPubMed
8.Minga, U, Wray, C. Serological tests for Salmonella enteritidis in chickens. Vet Rec 1990; 126: 20–1.Google Scholar
9.Barrow, PA, Lovell, MA. Experimental infection of egg-laying hens with Salmonella enteritidis phage type 4. Avian Pathol 1991; 20: 339–52.Google ScholarPubMed
10.Nicholas, RAJ, Cullen, GA. Development and application of an ELISA for detecting antibodies to Salmonella enteritidis in chicken flocks. Vet Rec 1991; 128: 74–6.CrossRefGoogle ScholarPubMed
11.Hassan, JO, Mockett, APA, Catty, D, Barrow, PA. Infection and reinfection of chickens with Salmonella typhimurium: bacteriology and immune responses. Avian Dis 1991; 35: In press.CrossRefGoogle ScholarPubMed
12.Nicholas, RAJ, Cullen, GA, Duff, P. Detection of Salmonella. Vet Rec 1990; 126: 147.Google ScholarPubMed
13.Chart, H, Rowe, B, Baskerville, A, Humphrey, TJ. Serological tests for Salmonella enteritidis in chickens. Vet Rec 1990; 129: 20.Google Scholar
14.Chart, H, Rowe, B, Baskerville, A, Humphrey, TJ. Serological tests for Salmonella enteritidis in chickens. Vet Rec 1990; 126: 92.Google ScholarPubMed
15.Chart, H, Rowe, B, Baskerville, A, Humphrey, TJ. Serological analysis of chicken flocks for antibodies to Salmonella enteritidis. Vet Rec 1990; 127: 501–2.Google ScholarPubMed
16.Chart, H, Rowe, B, Baskerville, A, Humphrey, TJ. Serological analysis for antibodies to S. enteritidis. Vet Rec 1991; 128: 215.CrossRefGoogle ScholarPubMed
17.Barrow, PA. Serological analysis for antibodies to S. enteritidis. Vet Rec 1991; 128: 43–4.CrossRefGoogle ScholarPubMed
18.Barrow, PA, Huggins, MB, Lovell, MA, Simpson, JM. Observations on the pathogenesis of experimental Salmonella typhimurium infection in chickens. Res Vet Sci 1987; 42: 194–9.CrossRefGoogle ScholarPubMed
19.Bumstead, N, Barrow, PA. Genetics of resistance to Salmonella typhimurium in newly hatched chicks. Br Poult Sci 1988; 29: 521–9.CrossRefGoogle ScholarPubMed
20.Smith, HW, Tucker, JF. The effect of antibiotic therapy on the faecal excertion of Salmonella typhimurium by experimentally infected chickens. J Hyg 1975; 75: 275–92.CrossRefGoogle Scholar
21.Barrow, PA, Simpson, JM, Lovell, MA. Intestinal colonization in the chicken by food-poisoning Salmonella serotypes; microbial characteristics associated with faecal excretion. Avian Path 1988; 17: 571–88.CrossRefGoogle ScholarPubMed
22.Lawn, AM. Comparison of the flagellins from different flagellar morphotypes of Escherichia coli. J Gen Microbiol 1977; 101: 112–30.CrossRefGoogle ScholarPubMed
23.Sambrook, J, Frisch, EF, Maniatis, J. Molecular cloning – a laboratory manual. 2nd ed.USA: Cold Spring Harbor Laboratory Press. 1989.Google Scholar
24.Barrow, PA, Berchieri, A, Al-Haddad, O. The serological response of chickens to infection with Salmonella gallinarum-pullorum detected by ELISA. Avian Dis 1991. In press.Google Scholar
25.Humphrey, TJ, Baskerville, A, Chart, H, Rowe, B. Infection of egg laying hens with Salmonella enteritidis PT4 by oral inoculation. Vet Rec 1989; 125: 531–2.CrossRefGoogle ScholarPubMed
26.Barrow, PA. Experimental infection of chickens with Salmonella enteritidis. AV Pathol 1991; 20: 145–53.CrossRefGoogle ScholarPubMed