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Cell surface hydrophobicity, adherence to HeLa cell cultures and haemagglutination pattern of pyelonephritogenic Escherichia coli strains

Published online by Cambridge University Press:  15 May 2009

A. Brauner ,
M. Katouli ,
K. Tullus  and
S. H. Jacobson
A. Brauner*
Affiliation:
Department of Clinical Microbiology, Stockholm County Council and Karolinska Hospital, Stockholm, Sweden
M. Katouli
Affiliation:
Department of Bacteriology, Karolinska Institute, Stockholm, Sweden
K. Tullus
Affiliation:
Department of Paediatrics, Danderyd Hospital, Stockholm, Sweden
S. H. Jacobson
Affiliation:
Department of Medicine, Division of Nephrology, Karolinska Hospital, Stockholm, Sweden
*
*Dr A. Brauner, Department of Clinical Microbiology, Stockholm County Council, PO Box 70470, S-107 26 Stockholm, Sweden.
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Cell surface hydrophobicity, haemagglutination pattern and adherence to HeLa cells were examined in 230 strains of Escherichia coli collected from women (n = 61 strains) and children (n = 65 strains) with non-obstructive acute pyelonephritis and in 104 faecal control strains of E. coli from healthy adults (n = 71 strains) and children (n = 33 strains). Pyelonephritogenic E. coli strains showed a significantly increased incidence of hydrophobic properties (90%) and mannose resistant haemagglutination (MRHA) of human erythrocytes (83%) than faecal control strains (64 and 23% respectively, P < 0·001 in both cases). Mannose sensitive haemagglutination (MSHA) was observed in 48% of the pyelonephritogenic E. coli strains and in 50% of the faecal control strains (NS). The incidence of adherence to HeLa cells was low both in pyelonephritogenic and faecal control strains, 6 and 7% respectively (NS). The bacterial phenotypes MRHA + MSHA + and MRHA + MSHA− appeared significantly more often in pyelonephritogenic E. coli strains (35 and 48% respectively) than in faecal control strains (5 and 17% respectively, P < 0·001 in both cases). The phenotype MRHA − MSHA + occurred significantly more often in control strains (45%) than in pyelonephritogenic strains (13%, P < 0·001). Eighty-three per cent of the pyelonephritogenic E. coli strains expressing hydrophobic properties showed MRHA and 50% of the hydrophobic strains showed MSHA. There were no significant correlations between cell surface hydrophobic properties and haemagglutination pattern or adherence to HeLa cells in pyelonephritogenic E. coli strains nor in faecal control strains.

Type
Research Article
Information
Epidemiology & Infection , Volume 105 , Issue 2 , October 1990 , pp. 255 - 263
Copyright
Copyright © Cambridge University Press 1990

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