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Diverse Mutations in the Acetolactate Synthase Gene Confer Chlorsulfuron Resistance in Kochia (Kochia scoparia) Biotypes

Published online by Cambridge University Press:  12 June 2017

Mary J. Guttieri ,
Charlotte V. Eberlein  and
Donald C. Thill
Mary J. Guttieri
Affiliation:
Univ. Idaho, Aberdeen, ID 83210
Charlotte V. Eberlein
Affiliation:
Univ. Idaho, Aberdeen, ID 83210
Donald C. Thill
Affiliation:
Univ. Idaho, Moscow, ID 83844
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Abstract

The DNA sequence of an 83-base pair region of the acetolactate synthase (ALS) gene was compared for 10 chlorsulfuron-resistant (R) and three chlorsulfuron-susceptible (S) kochia biotypes. Point mutation in the codon for the proline residue at position 173 in Domain A of the ALS protein was observed in seven of 10 R biotypes. Among these seven R biotypes, mutation to threonine, serine, arginine, leucine, glutamine, and alanine was identified. The mechanism of resistance was determined for the R biotypes that did not have mutation in Domain A; all were resistant due to modified ALS, which indicated that at least one non-Domain A mutation site for resistance exists in kochia. Sequence results indicate that multiple mutations for resistance have occurred, and that geographically widespread ALS-inhibitor resistance in kochia is not the result of a single resistance allele.

Keywords

Chlorsulfuron (2-chloro-N-[[(4-methoxy-6-methyl-1,3,5-triazin-2-yl)amino]carbonyl]benzenesulfonamide)acetolactate synthase (ALS; EC 4.1.3.18)kochia, Kochia scoparia L. (Schrad.) #3 KCHSCGenetic variationherbicide resistanceKCHSC
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 43 , Issue 2 , June 1995 , pp. 175 - 178
Copyright
Copyright © 1995 by the Weed Science Society of America 

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References

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