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Action of Trifluralin on Chromatin Activity in Corn and Soybean

Published online by Cambridge University Press:  12 June 2017

Donald Penner
Affiliation:
Dep. of Crop and Soil Sci., Mich. State Univ., East Lansing, Michigan 48823
Roy W. Early
Affiliation:
Dep. of Crop and Soil Sci., Mich. State Univ., East Lansing, Michigan 48823

Abstract

Trifluralin (α,α,α-trifluoro-2,6-dinitro-N,N-dipropyl-p-toluidine) at 10−5 M applied to etiolated corn (Zea mays L. ‘Michigan 500′) seedlings 6 or 12 hr before the isolation of chromatin from the roots markedly reduced ribonucleic acid (RNA) synthesis supported by the chromatin. The addition of Escherichia coli RNA polymerase failed to overcome the inhibition. Trifluralin increased the melting temperature of the chromatin. The presence of trifluralin during the isolation and reaction procedure inhibited RNA synthesis indicating possible trifluralin binding to the chromatin with subsequent reduction of template availability for transcription. Trifluralin did not inhibit chromatin activity in soybean [Glycine max (L.) Merr. ‘Hark’] seedlings.

Type
Research Article
Copyright
Copyright © Weed Science Society of America 

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