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The high resolution melting analysis (HRM) as a molecular tool for monitoring parasites of the wildlife

Published online by Cambridge University Press:  12 December 2016

LAURENT HÉRITIER ,
OLIVIER VERNEAU ,
GREGORY BREUIL  and
ANNE-LEILA MEISTERTZHEIM
LAURENT HÉRITIER*
Affiliation:
Univ. Perpignan Via Domitia, CEntre de Formation et de Recherche sur les Environnements Méditerranéens, UMR 5110, F-66860, Perpignan, France CNRS, CEntre de Formation et de Recherche sur les Environnements Méditerranéens, UMR 5110, F-66860, Perpignan, France Unit for Environmental Sciences and Management, North-West University, Potchefstroom, 2520, South Africa
OLIVIER VERNEAU
Affiliation:
Univ. Perpignan Via Domitia, CEntre de Formation et de Recherche sur les Environnements Méditerranéens, UMR 5110, F-66860, Perpignan, France CNRS, CEntre de Formation et de Recherche sur les Environnements Méditerranéens, UMR 5110, F-66860, Perpignan, France Unit for Environmental Sciences and Management, North-West University, Potchefstroom, 2520, South Africa
GREGORY BREUIL
Affiliation:
Clinique vétérinaire des NAC, 17 impasse Teynier, 31100, Toulouse, France
ANNE-LEILA MEISTERTZHEIM
Affiliation:
Univ. Perpignan Via Domitia, CEntre de Formation et de Recherche sur les Environnements Méditerranéens, UMR 5110, F-66860, Perpignan, France CNRS, CEntre de Formation et de Recherche sur les Environnements Méditerranéens, UMR 5110, F-66860, Perpignan, France
*
*Corresponding author: Univ. Perpignan Via Domitia, CEntre de Formation et de Recherche sur les Environnements Méditerranéens, UMR 5110, F-66860, Perpignan, France, CNRS, CEntre de Formation et de Recherche sur les Environnements Méditerranéens, UMR 5110, F-66860, Perpignan, France and Unit for Environmental Sciences and Management, North-West University, Potchefstroom, 2520, South Africa. E-mail: laurent.heritier@univ-perp.fr
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Summary

In an interconnected world, the international pet trade on wild animals is becoming increasingly important. As a consequence, non-native parasite species are introduced, which affect the health of wildlife and contribute to the loss of biodiversity. Because the investigation of parasite diversity within vulnerable host species implies the molecular identification of large samples of parasite eggs, the sequencing of DNA barcodes is time-consuming and costly. Thereby, the objectives of our study were to apply the high resolution melting (HRM) approach for species determination from pools of parasite eggs. Molecular assays were validated on flatworm parasites (polystomes) infecting the Mediterranean pond turtle Mauremys leprosa and the invasive red-eared slider Trachemys scripta elegans in French natural environments. HRM analysis results indicated that double or multiple parasitic infections could be detected from wild animal populations. They also showed that the cycle of parasite eggs production was not regular over time and may depend on several factors, among which the ecological niche and the target species. Thereby, monitoring parasites from wild endangered animals implies periodic parasitological surveys to avoid false negative diagnostics, based solely on eggs production.

Keywords

HRM12S rRNA genepolystomeMauremys leprosaTrachemys scripta elegansendangered wildlife
Type
Research Article
Information
Parasitology , Volume 144 , Issue 5 , April 2017 , pp. 563 - 570
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Copyright
Copyright © Cambridge University Press 2016 

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