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Distinctive histopathology and modulation of cytokine production during oral and intraperitoneal Trypanosoma cruzi Y strain infection

Published online by Cambridge University Press:  19 February 2014

CHRISTIAN C. KUEHN ,
LUIZ GUSTAVO R. OLIVEIRA ,
MARIZA ABREU MIRANDA  and
JOSÉ CLÓVIS PRADO Jr.
CHRISTIAN C. KUEHN*
Affiliation:
Faculdade de Ciências Farmacêuticas de Ribeirão Preto FCFRP-USP, Universidade de São Paulo, Avenida do Café, s/n, 14040-903, Ribeirão Preto, SP, Brazil
LUIZ GUSTAVO R. OLIVEIRA
Affiliation:
Faculdade de Ciências Farmacêuticas de Ribeirão Preto FCFRP-USP, Universidade de São Paulo, Avenida do Café, s/n, 14040-903, Ribeirão Preto, SP, Brazil
MARIZA ABREU MIRANDA
Affiliation:
Faculdade de Ciências Farmacêuticas de Ribeirão Preto FCFRP-USP, Universidade de São Paulo, Avenida do Café, s/n, 14040-903, Ribeirão Preto, SP, Brazil
JOSÉ CLÓVIS PRADO Jr.
Affiliation:
Faculdade de Ciências Farmacêuticas de Ribeirão Preto FCFRP-USP, Universidade de São Paulo, Avenida do Café, s/n, 14040-903, Ribeirão Preto, SP, Brazil
*
*Corresponding author: Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto FCFRP-USP, Universidade de São Paulo, Avenida do Café s/n°, 14040-903, Ribeirão Preto, SP, Brazil. E-mail: biochris@fcfrp.usp.br
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Summary

Acute Chagas disease outbreaks are related to the consumption of food or drink contaminated by triatomine feces, thus making oral infection an important route of transmission. Both vector-borne and oral infections trigger important cardiac manifestations in the host that are related to a dysregulated immune response. The aims of this work were to evaluate possible alterations of lymphocyte CD4+/CD8+ sub-populations, Th1 and Th2 cytokines, nitrite concentrations and cardiac histopathology. One group of male Wistar rats was intraperitoneally infected (I.P.) with 1×105 metacyclic trypomastigotes of the T. cruzi Y strain, and another group of Wistar rats was orally infected (O.I.) with 8×105 metacyclic trypomastigotes of the same strain. The intraperitoneal infection triggered statistically enhanced parasite and peritoneal macrophage numbers, increased concentrations of NO and IL-12 and elevated cardiac inflammatory foci when compared with the oral infection. However, proliferation of CD4+ and CD8+ T cells were not statistically different for oral and intraperitoneal routes.

Keywords

Intraperitoneal and oral infection routeTrypanosoma cruzi (Y strain)parasitaemianitric oxideInterleukin Th-1/Th-2flow cytometryhistopathology
Type
Research Article
Information
Parasitology , Volume 141 , Issue 7 , June 2014 , pp. 904 - 913
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Copyright
Copyright © Cambridge University Press 2014 

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