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DNA synthesis in gametocytes of Plasmodium falciparum

Published online by Cambridge University Press:  06 April 2009

C. J. Janse ,
T. Ponnudurai ,
A. H. W. Lensen ,
J. H. E. Th. Meuwissen ,
J. Ramesar ,
M. Van Der Ploeg  and
J. P. Overdulve
C. J. Janse
Affiliation:
Department of Tropical Veterinary Medicine and Protozoology, University of Utrecht, P.O. Box 80.172, 3508 TD Utrecht
T. Ponnudurai
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, Geert Grooteplein Zuid 24 6525 GA Nijmegen, The Netherlands
A. H. W. Lensen
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, Geert Grooteplein Zuid 24 6525 GA Nijmegen, The Netherlands
J. H. E. Th. Meuwissen
Affiliation:
Institute of Medical Parasitology, University of Nijmegen, Geert Grooteplein Zuid 24 6525 GA Nijmegen, The Netherlands
J. Ramesar
Affiliation:
Laboratory of Parasitology (Medical Faculty), University of Leiden, Wassenaarseweg 62, P.O. Box 9605, 2300 RC Leiden, The Netherlands
M. Van Der Ploeg
Affiliation:
Department of Histochemistry and Cytochemistry, University of Leiden, Wassenaarseweg 72, P.O. Box 9503, 2300 RA Leiden, The Netherlands
J. P. Overdulve
Affiliation:
Department of Tropical Veterinary Medicine and Protozoology, University of Utrecht, P.O. Box 80.172, 3508 TD Utrecht
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Summary

The DNA content of Plasmodium falciparum gametocytes during intra-erythrocytic development and during gametogenesis was established by cytophotometric methods. Intra-erythrocytic micro- and macrogametocytes (Stage I-Stage VB) contain about twice the amount of DNA of haploid sporozoites and ringstages, indicating that DNA is synthesized during transformation of ringforms into Stage I gametocytes. Microgametocytes, after activation at pH 8, rapidly duplicate their genome several times, while the DNA content of macrogametocytes remains constant during gametogenesis.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 1 , February 1988 , pp. 1 - 7
Copyright
Copyright © Cambridge University Press 1988

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References

REFERENCES

Cornelissen, A. W. C. A., Overdulve, J. P. & Van Der Ploeg, M. (1984). Determination of nuclear DNA of five eucoccidian parasites, Isospora (Toxoplasma) gondii, Sarcocystis cruzi, Eimeria tenella, E. acervulina and Plasmodium berghei, with special reference to gametogenesis and meiosis in I. (T.) gondii. Parasitology 88, 531–53.CrossRefGoogle Scholar
Dore, E., Birago, C., Frontali, C. & Battaglia, P. A. (1980). Kinetic complexity and repetitivity of Plasmodium berghei DNA. Molecular and Biochemical Parasitology 1, 199–208.CrossRefGoogle Scholar
Janse, C. J., Mons, B., Rouwenhorst, R. J., Van Der Klooster, P. F. J., Overdulve, J. P. & Van Der Kaay, H. J. (1985). In vitro formation of ookinetes and functional maturity of Plasmodium berghei gametocytes. Parasitology 91, 19–29.CrossRefGoogle ScholarPubMed
Janse, C. J., Van Der Klooster, P. F. J., Van der Kaay, H. J., Van der Ploeo, M. & Overdulve, J. P. (1986 a). Rapid repeated DNA replication during microgametogenesis and DNA synthesis in young zygotes of Plasmodium berghei. Transactions of the Royal Society of Tropical Medicine and Hygiene 80, 154–7.CrossRefGoogle ScholarPubMed
Janse, C. J., Van der Klooster, P. F. J., Van der Kaay, H. J., Van der Ploeg, M. & Overdulve, J. P. (1986 b). DNA synthesis in Plasmodium berghei during asexual and sexual development. Molecular and Biochemical Parasitology 20, 173–83.CrossRefGoogle ScholarPubMed
Janse, C. J., Van der Klooster, P. F. J., Van der Kaay, H. J., Van der Ploeg, M. & Overdulve, J. P. (1986 c). Mitomycin-C is an unreliable inhibitor for study of DNA synthesis in Plasmodium. Molecular and Biochemical Parasitology 21, 33–6.CrossRefGoogle ScholarPubMed
Janse, C. J., Van Vianen, P. H., Tanke, H. J., Mons, B., Ponnudurai, T. & Overdulve, J. P. (1987). Plasmodium species: Flow cytometry and microfluorometry assessments of DNA content and synthesis. Experimental Parasitology 64, 88–94.CrossRefGoogle ScholarPubMed
Mons, B., Janse, C. J., Boorsma, E. G. & Van der Kaay, H. J. (1985). Synchronized erythrocytic schizogony and gametocytogenesis of Plasmodium berghei in vivo and in vitro. Parasitology 91, 423–30.CrossRefGoogle ScholarPubMed
Ponnudurai, T., Lensen, A. H. W., Meis, J. F. G. M. & Meuwissen, J. H. E. Th. (1986). Sychronization of Plasmodium falciparum gametocytes using an automated suspension culture system. Parasitology 93, 263–74.CrossRefGoogle Scholar
Sinden, R. E. (1982). Gametocytogenesis of Plasmodium falciparum in vitro: an electron microscope study. Parasitology 84, 1–11CrossRefGoogle Scholar
Sinden, R. E. (1983). Sexual development of malarial parasites. Advances in Parasitology 22, 153–216.CrossRefGoogle ScholarPubMed
Toyé, P. J., Sinden, R. E. & Canning, E. U. (1977). The action of metabolic inhibitors on microgametogenesis in Plasmodium yoelii nigeriensis. Zeitschrift für Parasitenkunde 53, 133–41.CrossRefGoogle ScholarPubMed