Hostname: page-component-848d4c4894-ttngx Total loading time: 0 Render date: 2024-05-27T03:18:09.832Z Has data issue: false hasContentIssue false
  • English
  • Français

The ENZYMEBA test: detection of intestinal Entamoeba histolytica infection by immuno-enzymatic detection of histolysain

Published online by Cambridge University Press:  06 April 2009

A. L. Luaces ,
T. Picó  and
A. J. Barrett
A. L. Luaces
Affiliation:
Departamento de Parasitología, Instituto de Medicina Tropical‘Pedro Kouri’ (IPK), Ciudad de la Habana, Cuba
T. Picó
Affiliation:
Departamento de Parasitología, Instituto de Medicina Tropical‘Pedro Kouri’ (IPK), Ciudad de la Habana, Cuba
A. J. Barrett
Affiliation:
Departament of Biochemistry, Strangeways Research Laboratory, Cambridge CBI 4RN, England
Get access Rights & Permissions [Opens in a new window]

Summary

We describe a new, improved test for the detection of intestinal infection by Entamoeba histolytica. The test depends upon immunoadsorption of the E. histolytica cysteine proteinase, histolysain, from faecal samples, and subsequent visual detection of the enzyme by a colour reaction. With samples from 200 volunteers, results agreed closely with those obtained by the conventional microscopic technique, and there were no false positive reactions with samples containing other parasites. The test is suitable for use either in the laboratory or in the field.

Keywords

Entamoeba histolyticahistolysainhistolysindiagnosisamoebiasisENZYMEBA
Type
Research Article
Information
Parasitology , Volume 105 , Issue 2 , October 1992 , pp. 203 - 205
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Barrett, A. J. (1972). A new assay for cathepsin B1 and other thiol proteinases. Analytical Biochemistry 47, 280–93.Google ScholarPubMed
Baumann, D. & Gottstein, B. (1987). A double-antibody sandwich ELISA for the detection of Entamoeba histolytica antigen in stool samples of humans. Tropical Medicine and Parasitology 38, 81–5.Google ScholarPubMed
Grundy, M. S. (1982). Preliminary observations using a multilayer ELISA method for the detection of Entamoeba histolytica trophozoite antigens in stool samples. Transactions of the Royal Society of Tropical Medicine and Hygiene 76, 396–400.CrossRefGoogle ScholarPubMed
Luaces, A. L. & Barrett, A. J. (1988). Affinity purification and biochemical characterization of histolysin: the cysteine proteinase of Entamoeba histolytica. The Biochemical Journal 250, 903–9.CrossRefGoogle ScholarPubMed
Mathur, T. N. & Kaur, J. (1973). The frequency of excretion of cysts of Entamoeba histolytica in known cases of non-dysenteric amoebic colitis based on 21 stool examinations. Indian Journal of Medical Research 61, 330–3.Google ScholarPubMed
Ungar, B. L. P., Yolken, R. H. & Quinn, T. C. (1985). Use of monoclonal antibody in an enzyme immunoassay for the detection of Entamoeba histolytica in fecal specimens. American Journal of Tropical Medicine and Hygiene 34, 46572.CrossRefGoogle Scholar
Walsh, J. A. (1986). Problems in recognition and diagnosis of amebiasis: estimation of the global magnitude of morbidity and mortality. Revue of Infectious Diseases 8, 228–38.CrossRefGoogle ScholarPubMed