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An ultrastructural examination of irradiated, immunizing schistosomula of Schistosoma mansoni during their extended stay in the lungs

Published online by Cambridge University Press:  06 April 2009

Avis Mastin ,
Q. D. Bickle  and
R. A. Wilson
Avis Mastin
Affiliation:
Department of Biology, University of York, York YO1 5DD
Q. D. Bickle
Affiliation:
Winches Farm Field Station, London School of Hygiene and Tropical Medicine, Hatfield Road, St Albans, Herts.
R. A. Wilson
Affiliation:
Department of Biology, University of York, York YO1 5DD
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Extract

An ultrastructural study has been performed on radiation-attenuated parasites fixed in situ during their extended stay in the lungs. At day 7 post-infection all parasites were located within blood vessels. In contrast to normally migrating parasites, at days 13 and 21 post-infection the majority of radiation-attenuated parasites were lying free within alveoli. As far as could be seen, radiation-attenuated parasites had undergone the normal develop mental changes associated with the lung stage of migration. These included elongation of the body and loss of mid-body spines and the fibrous layer beneath the tegument. No ultrastructural evidence was found to suggest why schistosomula failed to migrate further and instead burst into alveoli. No parasites observed on day 7 post-infection were associated with a host inflammatory reaction. At day 13, inflammation was noticeable but could have been a response as much to pulmonary tissue damage as to the presence of parasites. At day 21 post-infection some parasites were internally disrupted but were not associated with host inflammatory reactions and had intact tegumental surfaces – all facts consistent with death by autophagy. One parasite was observed at day 21 with an adherent neutrophil infiltrate and extensive tegumental damage. The relevance of persisting lung-stage parasites to the induction of resistance to challenge is discussed.

Type
Research Article
Information
Parasitology , Volume 91 , Issue 1 , August 1985 , pp. 101 - 110
Copyright
Copyright © Cambridge University Press 1985

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References

REFERENCES

Bickle, Q. D., Dosiusou, T. & James, E. R. (1979 a). The effects of gamma-irradiation on migration and survival of Schistosoma mansoni schistosomula in mice. Parasitology 79, 223–30.Google Scholar
Bickle, Q. D., Taylor, M. G., Doenhoff, M. J. & Nelson, O. S. (1979 b). Immunization of mice with gamma-irradiated intramuscularly injected schistosomula of Schistosoma mansoni. Parasitology 79, 209–22.CrossRefGoogle ScholarPubMed
Crabtree, J. E. (1982). An ultrastructural study of the migrating schistosomulum of Schistosoma mansoni. D.Phil. thesis, University of York.Google Scholar
Crabtree, J. E. & Wilson, R. A. (1980). Schistosoma mansoni: a scanning electron microscope study of the developing schistosomulum. Parasitology 81, 553–64.Google Scholar
Hockley, D. J. & Mclaren, D. J. (1973). Schistosoma mansoni: changes in the membrane of the tegument during development from cercaria to adult worm. International Journal for Parasitology 3, 13–25.CrossRefGoogle ScholarPubMed
Hsu, S. Y. L., Hsu, H. F. & Burmeister, L. F. (1981). Schistosoma mansoni – vaccination of mice with highly irradiated cercariae. Experimental Parasitology 52, 91–104.CrossRefGoogle Scholar
Kay, D. (1965). Techniques for Electron Microscopy. Oxford & Edinburgh: Blackwell.Google Scholar
Mclaren, D. J., Hockley, D. J., Goldrino, O. L. & Hammond, B. J. (1978). A freeze fracture study of the developing tegumental outer membrane of Schistosoma mansoni. Parasitology 76, 327–48.CrossRefGoogle ScholarPubMed
Mangold, B. L. & Dean, D. A. (1984). The migration and survival of gamma-irradiated Schistosoma mansoni larvae and the duration of host–parasite contact in relation to the induction of resistance in mice. Parasitology 88, 249–66.Google Scholar
Mastin, A. J., Bickle, Q. D. & Wilson, R. A. (1983). Schistosoma mansoni: migration and attrition of irradiated and challenge schistosomula in the mouse. Parasitology 87, 87–102.Google Scholar
Miller, P. & Wilson, R. A. (1980). Migration of the schistosomula of Schistosoma mansoni from the lungs to the hepatic portal system. Parasitology 80, 267–88.Google Scholar
Minard, P., Dean, D. A., Jacobsen, R. H., Vannier, W. E. & Murrell, K. D. (1978). Immunization of mice with Cobalt-69 irradiated Schistosoma mansoni cercariae. American Journal of Tropical Medicine and Hygiene 27, 76–86.CrossRefGoogle ScholarPubMed
Smithers, S. R. & Terry, R. J. (1965). The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Wheater, P. R. & Wilson, R. A. (1979). Schistosoma mansoni: a histological study of migration in the laboratory mouse. Parasitology 79, 49–62.Google Scholar
Wilson, R. A., Draskau, T., Miller, P. & Lawson, J. R. (1978). Schistosoma mansoni: the activity and development of the schistosomulum during migration from the skin to the hepatic portal system. Parasitology 77, 57–73.CrossRefGoogle Scholar