Hostname: page-component-848d4c4894-ndmmz Total loading time: 0 Render date: 2024-05-21T02:03:30.965Z Has data issue: false hasContentIssue false
  • English
  • Français

Culture of Plasmodium falciparum in vitro: a subculture technique used for demonstrating antiplasmodial activity in serum from some Gambians, resident in an endemic malarious area

Published online by Cambridge University Press:  06 April 2009

R. S. Phillips ,
P. I. Trigg ,
T. J. Finnigan-Scott  and
R. K. Bartholomew
R. S. Phillips
Affiliation:
National Institute for Medical Research, London NW7 1AA
P. I. Trigg
Affiliation:
National Institute for Medical Research, London NW7 1AA
T. J. Finnigan-Scott
Affiliation:
National Institute for Medical Research, London NW7 1AA
R. K. Bartholomew
Affiliation:
Medical Research Council Laboratories, Fajara, The Gambia, West Africa
Get access Rights & Permissions [Opens in a new window]

Extract

A technique is described for obtaining consistent reinvasion and multiplication of the erythrocytic stages of Plasmodium falciparum from humans in vitro. Subsequent to this, P. falciparum was subcultured through almost three cycles in vitro, during which multiplication of the parasite was obtained in the first but maintenance or only slight increase in numbers was obtained in the second subculture. The effect of sera from adult Gambians on the reinvasion of P. falciparum was measured in vitro both morphologically and by the incorporation of [14C]isoleucine into parasite protein and by the incorporation of [3H]adenosine into parasite DNA and RNA. Two sera reduced the amount of reinvasion.

Type
Research Article
Information
Parasitology , Volume 65 , Issue 3 , December 1972 , pp. 525 - 535
Copyright
Copyright © Cambridge University Press 1972

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Avinsen, C. B, Geiman, Q. M, McKee, R. W, Ormsbee, R. A. & Ball, E. G. (1946). Studies on malarial parasites. VIII. Factors affecting the growth of Plasmodium knowlesi in vitro. Journal of Experimental Medicine 84, 607–21.Google Scholar
Bass, C. L. (1911). A new conception of immunity. Its application to the cultivation of protozoa,: and bacteria from the blood and to therapeutic measures. Journal American Medical Association 57, 1534–5.CrossRefGoogle Scholar
Black, R. H. (1946). The behaviour of New Guinea strains of Plasmodium falciparum and Plasmodium vivax when cultivated in vitro. Medical Journal of Australia 2, 109–11.CrossRefGoogle ScholarPubMed
Brown, K. N. & Brown, I. N. (1965). Immunity to malaria: antigen variation in chronic infections of Plasmodium knowlesi. Nature, London 208, 1286–8.CrossRefGoogle ScholarPubMed
Brown, I. N., Brown, K. N., Trigg, P. I., Phillips, R. S. & Hills, L. A. (1968). The effect of antiserum on the erythrocytic stage of Plasmodium knowlesi in vivo and in vitro. Parasitology 58, 17 P.Google Scholar
Brown, I. N., Brown, K. N., Trigg, P. I., Phillips, R. S. & Hills, L. A. (1970). Immunity to malaria. II. Serological response of monkeys sensitized by drug-suppressed infection or by dead parasitized cells in Freund's complete adjuvant. Experimental Parasitology 28, 318–38.CrossRefGoogle ScholarPubMed
Cohen, S., McGregob, I. A. & Carrington, S. P. (1961). Gamma-globulin and acquired immunity to human malaria. Nature, London 192, 733–7.CrossRefGoogle ScholarPubMed
Cohen, S. & Butcher, G. A. (1969). Comments on immunization. Military Medicine 131 (Supplement), 1191–7.CrossRefGoogle Scholar
Cohen, S. & Butcher, G. A. (1970). Properties of protective malaria antibody. Immunology 19, 369–83.Google Scholar
Cohen, S., Butcher, G. A. & Crandall, R. B. (1969). Action of malarial antibody in vitro. Nature, London 223, 368–71.CrossRefGoogle ScholarPubMed
Diggs, C. L., Pavanand, K., Permpanich, B., Numsuwankijkul, V., Haupt, R. & Chuanak, N. (1971). Penetration of human foetal erythrocytes by Plasmodium falciparum in vitro. Journal of Parasitology 57, 187–8.CrossRefGoogle ScholarPubMed
Geiman, Q. M., Siddiqui, W. A. & Schnell, J. V. (1966). In vitro studies on erythrocytic stages of plasmodia: medium improvement and results with seven species of malarial parasites. Military Medicine 131 (Supplement), 1015–25.CrossRefGoogle ScholarPubMed
Gutteridge, W. E. & Trigg, P. I. (1970). Incorporation of radioactive precursors into DNA and RNA of Plasmodium knowlesi in vitro. Journal of Protozoology 17, 89–96.CrossRefGoogle ScholarPubMed
Krebs, H. A. & Eggleston, L. V. (1940). The oxidation of pyruvate in pigeon breast muscle. Biochemical Journal 34, 442–51.CrossRefGoogle ScholarPubMed
McGregor, I. A., Carrington, S. P. & Cohen, S. (1963). Treatment of East African Plasmodium falciparum malaria with West African human γ-globulin. Transactions of the Royal Society of Tropical Medicine and Hygiene 57, 170–5.CrossRefGoogle Scholar
McGregor, I. A., Hall, P. J., Williams, K., Hardy, C. L. S. & Turner, M. W. (1966). Demonstration of circulating antibodies to Plasmodium falciparum by gel diffusion techniques. Nature, London 210, 1384–6.CrossRefGoogle ScholarPubMed
McGregor, I. A. & Wilson, R. J. M. (1971). Precipitating antibodies and immunoglobulins in Plasmodium falciparum infections in The Gambia. Transactions of the Royal Society of Tropical Medicine and Hygiene 65, 136–51.CrossRefGoogle ScholarPubMed
Siddiqui, W. A., Geiman, Q. M. & Schnell, J. V.. (1970). Use of a commercially available culture medium to test the susceptibility of human malarial parasites to antimalarial drugs. Journal of Parasitology 56, 188–9.CrossRefGoogle Scholar
Siddiqui, W. A., Schnell, J. V. & Geiman, Q. M.. (1970). In vitro cultivation of Plasmodium falciparum. American Journal of Tropical Medicine and Hygiene 19, 586–91.CrossRefGoogle ScholarPubMed
Trager, W.. (1966). Coenzyme A and the antimalarial action in vitro of antipantothenates against Plasmodium lophurae, P. coatneyi and P. falciparum. Transactions of the New York Academy of Science, Series II, 28, 1094–1108.CrossRefGoogle Scholar
Trager, W. (1971). A new method for intraerythrocytic cultivation of malaria parasites (Plasmodium coatneyi and P. falciparum). Journal of Protozoology 18, 239–42.CrossRefGoogle ScholarPubMed
Trigg, P. I. (1969). The use of proprietary tissue-culture media for the cultivation in vitro of the erythrocytic stages of Plasmodium knowlesi. Parasitology 59, 925–35.CrossRefGoogle ScholarPubMed
Trigg, P. I., Gutteridge, W. E. & Williamson, J. (1971). The effects of cordycepin on malarial parasites. Transactions of the Royal Society of Tropical Medicine and Hygiene 65, 514–20.CrossRefGoogle Scholar
Voller, A. (1971). Variant specific schizont agglutination antibodies in human malaria (Plasmodium falciparum) infections in Aotus. Transactions of the Royal Society of Tropical Medicine 65, 2–3.Google ScholarPubMed