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Phylogenetic analysis of partial mitochondrial cytochrome oxidase c subunit I and large ribosomal RNA sequences and nuclear internal transcribed spacer I sequences from species of Cyathostominae and Strongylinae (Nematoda, Order Strongylida), parasites of the horse

Published online by Cambridge University Press:  27 April 2001

A. McDONNELL ,
S. LOVE ,
A. TAIT ,
J.R. LICHTENFELS  and
J.B. MATTHEWS
A. McDONNELL
Affiliation:
Department of Veterinary Parasitology, University of Glasgow Veterinary School, Bearsden Road, G61 1 QH
S. LOVE
Affiliation:
Weipers Centre for Equine Welfare, University of Glasgow Veterinary School, Bearsden Road, G61 1 QH
A. TAIT
Affiliation:
Department of Veterinary Parasitology, University of Glasgow Veterinary School, Bearsden Road, G61 1 QH
J.R. LICHTENFELS
Affiliation:
Biosystematics and National Parasite Collection Unit, Agricultural Research Service, US Department of Agriculture, Beltsville, Maryland, 20705, USA
J.B. MATTHEWS
Affiliation:
Department of Veterinary Parasitology, University of Glasgow Veterinary School, Bearsden Road, G61 1 QH Division of Equine Studies, Department of Veterinary Clinical Science and Animal Husbandry, Faculty of Veterinary Science, University of Liverpool, Leahurst, Neston, CH64 7TE
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Abstract

Three nucleotide data sets, one nuclear (ITS-2) and two mitochondrial (COI and l-rRNA), have been investigated in order to determine relationships among species of Strongylinae and Cyathostominae, intestinal parasites of the horse.Nucleotide sequence data reported in this paper are available in the NCBI, GenBank, accession numbers: AF263472 to AF263472 and AF268479. The data exhibited a strong mutational bias towards A and T and in the COI gene, silent sites appeared to saturate rapidly partly due to this substitution bias. Thus, the COI gene was found to be less phylogenetically informative than the l-rRNA and ITS-2 genes. Combined analysis of the l-rRNA and ITS-2 genes supported a monophyletic clade of the cyathostomes with Tridentoinfundibulum gobi, which had previously been classified as a nematode of ‘uncertain origin'. The Strongylinae grouped consistently outside the clade containing the cyathostomes and T. gobi. Molecular analysis failed to provide strong evidence for the separation of cyathostomes into classical genera, as previously defined by morphological classification.

Keywords

horse strongylesStrongylinaeCyathostominaemolecular phylogenysubstitution biascombined analysis
Type
Research Article
Information
Parasitology , Volume 121 , Issue 6 , December 2000 , pp. 649 - 659
Copyright
2000 Cambridge University Press

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