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Distribution and significance of endothelin 1 in guinea pig cochlear lateral wall

Published online by Cambridge University Press:  26 February 2007

D-Y Xu ,
Y-D Tang ,
S-X Liu  and
J Liu
D-Y Xu
Affiliation:
Department of Otorhinolaryngology, West China Hospital of Sichuan University, Chengdu, China
Y-D Tang*
Affiliation:
Department of Otorhinolaryngology, West China Hospital of Sichuan University, Chengdu, China
S-X Liu
Affiliation:
Department of Otorhinolaryngology, West China Hospital of Sichuan University, Chengdu, China
J Liu
Affiliation:
Neural Science Center, West China Hospital of Sichuan University, Chengdu, China.
*
Address for correspondence: Yue-Di Tang, Department of Otorhinolaryngology, West China Hospital of Sichuan University, Chengdu, China 610041. E-mail: tangyd@hotmail.com
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Abstract

Endothelin 1 is a vasoconstrictive peptide with many biological functions. To investigate the distribution of endothelin 1 in guinea pig cochlear lateral wall and the significance of endothelin 1 in maintaining cochlear homeostasis, the immunohistochemistry avidin biotin complex method was applied by using rabbit anti-endothelin 1 polyclonal antibody as primary antibody. Endothelin-1-like activities were detected in the marginal cells, spiral prominence epithelial cells, outer sulcus cells, stria vascularis capillaries, basal cells and spiral ligament fibrocytes.

These results suggest that endothelin 1 may play an important role in maintaining cochlear homeostasis.

Keywords

Endothelin 1CochleaGuinea PigImmunohistochemistry
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 121 , Issue 8 , August 2007 , pp. 721 - 724
Copyright
Copyright © JLO (1984) Limited 2007

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References

1Yanagisawa, M, Kurihara, H, Kimura, S, Tomobe, Y, Kobayashi, M, Mitsui, Y et al. A novel potent vasoconstrictor peptide produced by vascular endothelial cells. Nature 1988;332:411–15CrossRefGoogle ScholarPubMed
2Jinnouchi, K, Tomiyama, S, Pawankar, S, Ikezono, T, Yagi, T. Distribution of endothelin-1-like activity in the cochlea of normal guinea pigs. Acta Otolaryngol 1997;117:41–5CrossRefGoogle ScholarPubMed
3Jinnouchi, K, Tomiyama, SI, Pawankar, R. Distribution of endothelin-1-like activity in the vestibule of normal guinea pigs. ORL J Otorhinolaryngol Relat Spec 1996;58:4–8CrossRefGoogle ScholarPubMed
4Kizawa, Y, Kotake, H, Kusama, T, Saito, K, Murakami, H. Antigen-induced elevation of immunoreactive endothelin-1 (ET-1) levels in ovalbumin-sensitized guinea pig airway tissue. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 1999;122:239–43CrossRefGoogle ScholarPubMed
5Goto, S, Oshima, T, Ikeda, K, Ueda, N, Takasaka, T. Expression and localization of the Na-K-2Cl cotransporter in the rat cochlea. Brain Res 1997;765:324–6CrossRefGoogle ScholarPubMed
6Nakazawa, K, Spicer, SS, Schulte, BA. Ultrastructural localization of Na,K-ATPase in the gerbil cochlea. J Histochem Cytochem 1995;43:981–91Google Scholar
7Marcus, DC, Chiba, T. K+ and Na+ absorption by outer sulcus epithelial cells. Hear Res 1999;134:48–56CrossRefGoogle ScholarPubMed
8Schulte, BA, Adams, JC. Distribution of immunoreactive Na+-K+-ATPase in gerbil cochlea. J Histochem Cytochem 1989;37:127–34CrossRefGoogle ScholarPubMed
9Prasanna, G, Dibas, A, Hulet, C, Yorio, T. Inhibition of Na(+)/K(+)-ATPase by endothelin-1 in human nonpigmented ciliary epithelial cells. J Pharmacol Exp Ther 2001;296:966–71Google Scholar
10Gupta, S, Ruderman, NB, Cragoe, EJ Jr, Sussman, I. Endothelin stimulates Na(+)-K(+)-ATPase activity by a protein kinase C-dependent pathway in rabbit aorta. Am J Physiol 1991;261(1Pt2):H38–45Google Scholar
11Chen, HX, Wang, JL, Liu, QC, Qiu, JH. Distribution and location of immunoreactive atrial natriuretic peptides in cochlear stria vascularis of guinea pig. Chin Med J (Engl) 1994;107:53–6Google Scholar
12Skvorak, JP, Nazian, SJ, Dietz, JR. Endothelin acts as a paracrine regulator of stretch-induced atrial natriuretic peptide release. Am J Physiol 1995;269(5Pt2):R1093–8Google ScholarPubMed