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The use of monoclonal antibodies in virology

Published online by Cambridge University Press:  25 March 2010

J. Oxford
J. Oxford
Affiliation:
National Institute for Biological Standards and Control, Holly Hill, Hampstead, London NW3 6RB
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Antibodies have been used for the last five decades in the laboratory diagnosis of a wide range of diseases caused by viruses and in detailed investigations of virus structure. However, immunological and serological assays have always had problems of interpretation, reproducibility and standardization, resulting partly from the unavoidable heterogeneity of the antibodies in the test. When a mouse, for example, is immunized with a virus, the animal may easily recognise 10–20 different antigenic determinants. As many as five distinct antibodies can be produced by the mouse against each determinant and these will often differ from antibodies made by another mouse against the same antigenic determinant. The method of lymphocyte fusion and the subsequent generation of monoclonal antibodies (Kohler & Milstein, 1975; Kohler, 1980) overcomes these limitations.

Type
Special Article
Information
Journal of Hygiene , Volume 88 , Issue 3 , June 1982 , pp. 361 - 368
Copyright
Copyright © Cambridge University Press 1982

References

REFERENCES

Air, G. M., Laver, W. G. & Webster, R. G. (1980). Towards a universal influenza vaccine. In New Developments with Human and Veterinary Vaccines (ed. Miztahi, A., Hertman, I., Klingberg, M. A. and Kohn, A.). New York: Alan R. Liss.Google Scholar
Birrer, M. J., Udem, S., Nathenson, S. & Bloom, B. R. (1981). Antigenic variants of measles virus. Nature 293, 67–69.CrossRefGoogle ScholarPubMed
Chakraverty, P., Cunningham, P. & Pereira, M. S. (1982). The return of the historic influenza A H1N1 virus and its impact on the population of the United Kingdom. Journal of Hygiene 89, (In the Press.)CrossRefGoogle ScholarPubMed
Cox, F. E. G. (1981). Hybridoma technology identifies protective malaria antigens. Nature 294, 612.CrossRefGoogle ScholarPubMed
Ferguson, M., Schild, G. C., Minor, P. D., Yates, P. J. & Spitz, M. (1981). A hybridoma cell line secreting antibody to poliovirus type 3 D antigen: detection in virus harvest of two D antigen populations. Journal of General Virology 54, 437–442.CrossRefGoogle ScholarPubMed
Gerhard, W. & Webster, R. G. (1978). Selection and characterisation of antigenic variants of A/PR/8/34 (H1N1) influenza virus with monoclonal antibodies. Journal of Experimental Medicine 148, 383–392.CrossRefGoogle ScholarPubMed
Giraudon, P. & Wild, T. F. (1981). Differentiation of measles virus strains and a strain of Canine Distemper Virus by monoclonal antibodies. Journal of General Virology 57, 179–183.CrossRefGoogle Scholar
Haaheim, L. R. (1980). Haemagglutination-inhibition antibodies in human sera to an antigenic mutant of influenza A/Texas/1/77 (H3N2) virus obtained in vitro. Acta Pathologica Scandinavica 88, 351–356.Google Scholar
Hochkeppel, H. K., Menge, U. & Collins, J. (1981). Monoclonal antibodies against human fibroblast interferon. Nature 291, 500–501.CrossRefGoogle ScholarPubMed
Icenogle, J., Gilbert, S. F., Grieves, J., Anderegg, J. & Rueckert, R. (1981). A neutralising monoclonal antibody against poliovirus and its reaction with related antigens. Virology 115, 211–215.CrossRefGoogle ScholarPubMed
Kennett, R. H., McKearn, T. J. & Bechtol, K. B. (Eds.) (1980). Monoclonal Antibodies. New York: Plenum.CrossRefGoogle Scholar
Kohler, G. & Milstein, C. (1975). Continuous cultures of fused cells secreting antibody of predefined specificity. Nature 256, 495–497.CrossRefGoogle ScholarPubMed
Kohler, G. (1980). Hybridoma techniques. Cold Spring Harbour Laboratory Manual.Google Scholar
Lostrom, M. E., Stone, M. R., Tam, M., Burnette, W. N., Pinter, A. & Nowinski, R. C. (1979). Monoclonal antibodies against murine leukaemia viruses; identification of six antigenic determinants on the p15(F) and gp70 envelope proteins. Virology 98, 336–350.CrossRefGoogle Scholar
Lubeck, M. D., Schulman, J. L. & Palese, P. (1980). Antigenic variants of influenza viruses. marked differences in the frequencies of variants selected with different monoclonal antibodies. Virology 102, 458–462.CrossRefGoogle ScholarPubMed
Massey, R. J. & Schochetman, G. (1981). Topographical analysis of viral epitopes using monoclonal antibodies: mechanism of virus neutralisation. Virology 115, 20–32.CrossRefGoogle Scholar
Natali, A., Oxford, J. S. & Schild, G. C. (1981). Frequency of naturally occurring antibody to influenza virus antigenic mutants selected in vitro with monoclonal antibody. Journal of Hygiene 87, 185–191.CrossRefGoogle ScholarPubMed
Pereira, M. S. & Chakraverty, P. (1982). Influenza in the United Kingdom 1977–81. Journal of Hygiene 88, 501–512.CrossRefGoogle Scholar
Russell, W. C., Patel, G., Precious, B., Sharp, I., & Gardner, P. S. (1981). Monoclonal antibodies against adenovirus type 5: preparation and preliminary classification. Journal of General Virology 56, 393–408.CrossRefGoogle Scholar
Secher, D. S. & Burke, D. C. (1980). A monoclonal antibody for large scale purification of human leukocyte interferon. Nature 285, 446–450.CrossRefGoogle ScholarPubMed
Shulman, M., Wilde, C. D. & Kohler, G. (1978). A better cell line for making hybridomas secreting specific antibodies. Nature 276, 269–270.CrossRefGoogle ScholarPubMed
Tedder, R. S., Yao, J. L. & Anderson, M. J. (1982). The production of monoclonal antibodies to rubella haemagglutinin and their use in antibody capture assays for rubella-specific IgM. Journal of Hygiene 88, 335–350.CrossRefGoogle ScholarPubMed
Van Wyke, K. L., Bean, W. J. & Webster, R. G. (1981). Monoclonal antibodies to the influenza A virus nucleoprotein affecting RNA transcription. Journal of Virology 39, 313–317.CrossRefGoogle Scholar
Yewdell, J. W. & Gerhard, W. (1981). Antigenic characterisation of viruses by monoclonal antibodies. Annual Review of Microbiology 35, 185–206.CrossRefGoogle ScholarPubMed
Yewdell, J. W., Webster, R. G. & Gerhard, W. U. (1979). Antigenic variation in three distinct determinants of an influenza type A haemagglutinin molecule. Nature 279, 246–248.CrossRefGoogle ScholarPubMed
Webster, R. G. & Berton, M. T. (1981). Analysis of antigenic drift in the haemagglutinin molecule of influenza B virus with monoclonal antibodies. Journal of General Virology 54, 243–251.CrossRefGoogle ScholarPubMed
Wiktor, T. J. & Koprowski, H. (1980). Antigenic variants of rabies virus. Journal of Experimental Medicine 152, 99–112.CrossRefGoogle ScholarPubMed
Wilson, I. A., Skehel, J. J. & Wiley, D. C. (1981). The haemagglutinin membrane glycoprotein of influenza virus: structure at 3 Å resolution. Nature 289, 366–373.CrossRefGoogle ScholarPubMed