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In vitro measurement of the potency of inactivated foot-and-mouth disease virus vaccines

Published online by Cambridge University Press:  15 May 2009

F. Brown  and
J. F. E. Newman
F. Brown
Affiliation:
Research Institute (Animal Virus Diseases), Pirbright, Surrey
J. F. E. Newman
Affiliation:
Research Institute (Animal Virus Diseases), Pirbright, Surrey
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Inactivated vaccines have been prepared from one strain of FMD virus grown in guinea-pig pad epithelium, unweaned mice and cultured pig kidney and baby hamster kidney cells. The potencies of these vaccines in protecting guinea-pigs against challenge with inoculated infective virus of the same strain have been compared and related to the amounts of 25 mμ component present in the different virus suspensions. Although it was possible to obtain a relationship between the content of 25 mμ component and potency for an individual source of virus, this relationship does not hold for all the different sources of virus used. It is suggested that the reason for this failure is the partial masking of the 25 mμ component by a cell constituent present in some of the virus suspensions so that the component is incompletely estimated by the complement-fixation test.

Type
Research Article
Information
Journal of Hygiene , Volume 61 , Issue 3 , September 1963 , pp. 345 - 351
Copyright
Copyright © Cambridge University Press 1963

References

REFERENCES

Beale, A. J. (1961). The D-antigen content in poliovaccine as a measure of potency. Lancet, ii, 1166.CrossRefGoogle Scholar
Brooksby, J. B. (1952). The technique of complement-fixation in foot-and-mouth disease research. Agric. Res. Coun. Rep. Ser. no. 12.Google Scholar
Brown, F. & Cartwright, B. (1960). Purification of the virus of foot-and-mouth disease by fluorocarbon treatment and its dissociation from neutralizing antibody. J. Immunol. 85, 309.Google Scholar
Brown, F., Cartwright, B. & Stewart, D. L. (1962). Further studies on the infection of pig-kidney cells by foot-and-mouth disease virus. Biochim. Biophys. Acta, 55, 768.CrossRefGoogle ScholarPubMed
Brown, F., Cartwright, B. & Stewart, D. L. (1963). The effect of various inactivating agents on the viral and ribonucleic acid infectivities of foot-and-mouth disease virus and on its attachment to susceptible cells. J. gen. Microbiol. 31, 444.CrossRefGoogle ScholarPubMed
Brown, F. & Crick, J. (1959). Application of agar-gel diffusion analysis to a study of the antigenic structure of inactivated vaccines prepared from the virus of foot-and-mouth disease. J. Immunol. 82, 444.CrossRefGoogle ScholarPubMed
Henderson, W. M. (1953). The use of virus culture in foot-and-mouth disease research. Proc. 15th Int. vet. Congr. 1, 206.Google Scholar
Holland, J. J. & McLaren, L. C. (1959). The mammalian cell-virus relationship. II. Adsorption, reception, and eclipse of polio-virus by HeLa cells J. exp. Med. 109, 487.Google Scholar
MacPherson, I. A. & Stoker, M. G. P. (1962). Polyoma transformation of hamster cell clones—an investigation of genetic factors affecting cell competence. Virology, 16, 147.Google Scholar
Mussgay, M. (1959). Trennung zweier spezifischer Einheiten des Virus der Maul-und-Klauenseuche mit einem Fluor-Kohlenwasserstoff. Z. Hyg. InfektKr., 146, 48.CrossRefGoogle Scholar
Randrup, A. (1954). On the stability of bovine foot-and-mouth disease virus dependent on pH. Investigations on the complement fixing and the immunising antigen as well as on the Infective agent. Acta path. microbiol. Scand. 35, 388.CrossRefGoogle ScholarPubMed
Skinner, H. H. (1951). Propagation of strains of foot-and-mouth disease virus in unweaned white mice. Proc. R. Soc. Med. 44, 1041.Google ScholarPubMed
Ubertini, B., Nardelli, L., Barei, S., & Santen, G. (1956). Études sur la culture in vitro du virus aphteux selon Frenkel. Zbl. vet. Med. 3, 419.Google Scholar