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Reduction of benzimidazole resistance in established Haemonchus contortus populations in goats using a single infection with a benzimidazole-susceptible isolate

Published online by Cambridge University Press:  16 September 2014

J.I. Chan-Pérez ,
J.F.J. Torres-Acosta ,
R.I. Rodríguez-Vivas  and
S.L. Villegas-Pérez
J.I. Chan-Pérez
Affiliation:
Campus de Ciencias Biológicas y Agropecuarias, FMVZ, Universidad Autónoma de Yucatán, Km 15.5 Carretera Mérida-Xmatkuil, Mérida, Yucatán, México
J.F.J. Torres-Acosta*
Affiliation:
Campus de Ciencias Biológicas y Agropecuarias, FMVZ, Universidad Autónoma de Yucatán, Km 15.5 Carretera Mérida-Xmatkuil, Mérida, Yucatán, México
R.I. Rodríguez-Vivas
Affiliation:
Campus de Ciencias Biológicas y Agropecuarias, FMVZ, Universidad Autónoma de Yucatán, Km 15.5 Carretera Mérida-Xmatkuil, Mérida, Yucatán, México
S.L. Villegas-Pérez
Affiliation:
Campus de Ciencias Biológicas y Agropecuarias, FMVZ, Universidad Autónoma de Yucatán, Km 15.5 Carretera Mérida-Xmatkuil, Mérida, Yucatán, México
*
*Fax: +52-999-942-32-05E-mail: tacosta@uady.mx
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Abstract

An in vivo study in goats evaluated the effect of superimposing a single artificial infection with a benzimidazole (BZ)-susceptible Haemonchus contortus isolate upon established H. contortus populations of known BZ resistance by measuring the phenotypic BZ resistance of eggs collected from faeces before and after re-infection. Two H. contortus isolates, one benzimidazole resistant (BZR) and the other susceptible (BZS), were used to infect worm-free goats. Eight goats were initially infected with 2000 third-stage larvae (L3). In each case the inoculum contained a pre-determined proportion of the two isolates: 100% BZS (one goat), 75% BZS/25% BZR (two goats), 50% BZS/50% BZR (two goats), 25%BZS/75% BZR (two goats) and, finally, 100% BZR (one goat). The phenotypic BZ susceptibility of the H. contortus population formed in each goat after the first infection was determined on days 30 and 36 post-infection using an egg-hatch assay (EHA) that estimated the concentration of thiabendazole (TBZ) required for 95% inhibition of larval hatching (EC95) with a 95% confidence interval (95% CI). On day 49 post-infection, goats were re-infected with 2000 L3 of the BZS isolate alone. A second set of EHA bioassays was performed 28 days and 34 days after re-infection. The first infection protocol produced three populations classified as BZS (EC95 0.055–0.065 μg TBZ/ml) while four were categorized as BZR (EC95 0.122–0.344 μg TBZ/ml). The status of one other population could not be determined. After re-infection with BZS L3, the number of susceptible populations increased to six (EC95 0.043–0.074 μg TBZ/ml) while the remaining two were deemed resistant (EC95 0.114–119 μg TBZ/ml). Re-infection with BZS L3 thereby reduced the resistance status of most H. contortus populations.

Type
Short Communications
Information
Journal of Helminthology , Volume 89 , Issue 5 , September 2015 , pp. 641 - 645
Copyright
Copyright © Cambridge University Press 2014 

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