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Influence of glycosylation on the hydration properties of caseinates

Published online by Cambridge University Press:  01 June 2009

Martine Le Meste ,
Bernard Colas ,
Geneviève Blond  and
Denise Simatos
Martine Le Meste
Affiliation:
Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Département de Biologie Physico-Chimique, Campus Universitaire, 21000 Dijon, France
Bernard Colas
Affiliation:
Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Département de Biologie Physico-Chimique, Campus Universitaire, 21000 Dijon, France
Geneviève Blond
Affiliation:
Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Département de Biologie Physico-Chimique, Campus Universitaire, 21000 Dijon, France
Denise Simatos
Affiliation:
Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation, Département de Biologie Physico-Chimique, Campus Universitaire, 21000 Dijon, France
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Summary

Glycosylation has been recognized as enhancing some functional properties of caseinates. The present work was aimed at elucidating the structure–function relationship of the functionality of caseinates and, more particularly, the influence of hydration on these properties. Measurements of non-frozen water content derived from sorption isotherms are presented. Using spin-probing Electron Spin Resonance we studied the mobilization pattern of solutes as hydration changed.The protein interactions with bound water were not significantly affected by sugar binding. Changes in the functional properties as, for example, in the water-holding capacity, reflected alterations in the structure, which was looser and more flexible after glycosylation, and in the associative properties (increased solubility) of caseinates. Disaccharides seemed more effective than monosaccharides in inducing these changes.

Type
Original Articles
Information
Journal of Dairy Research , Volume 56 , Issue 3: Special Issue: Proceedings of the Hannah Research Institute Casein Conference , May 1989 , pp. 479 - 486
Copyright
Copyright © Proprietors of Journal of Dairy Research 1989

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References

REFERENCES

Billmeyer, F. W. 1984 Text Book of Polymer Science 3rd ed.New York: WileyGoogle Scholar
Canton, M. C. & Mulvihill, D. M. 1983 In Physico-Chemical Aspects of Dehydrated Protein-rich Milk Products, pp. 339–353. Proceedings of IDF Symposium Helsingor, DenmarkGoogle Scholar
Colas, B., Gobin, C. & Lorient, D. 1988 Viscosity and voluminosity of caseins chemically modified by reductive alkylation with reducing sugars. Journal of Dairy Research 55 539–546CrossRefGoogle Scholar
Ferry, J. O. 1980 Viscoelastic Properties of Polymers 3rd ed.New York: WileyGoogle Scholar
Jowitt, R., Escher, F., Hallstrom, B., Meffert, M. F., Spiess, W. E. & Vos, G. 1983 Physical Properties of Foods. London: Applied Science PublishersGoogle Scholar
Kitabatake, N., Cuq, J. L. & Cheftel, J. L. 1985 Covalent binding of glycosyl residues to β-lactoglobulin: effects on solubility and heat stability. Journal of Agricultural and Food Chemistry 33 125–130CrossRefGoogle Scholar
Lee, H. S., Sen, L. C., Clifford, A. D., Whitaker, J. R. & Feeney, R. E. 1979 Preparation and nutritional properties of caseins covalently modified with sugars. Reductive alkylation of lysines with glucose, fructose or lactose. Journal of Agricultural and Food Chemistry 27 1094–1098CrossRefGoogle ScholarPubMed
Le Meste, M. & Duckworth, R. B. 1988 The influence of water content on the mobility of solute molecules and of protein side chains in Caseinate preparations. International Journal of Food Science and Technology 23 457–466CrossRefGoogle Scholar
Reilhmeier, R. A. F. 1985 In Biochemistry of Lipids and Membranes pp. 503–558 (Eds , D. E. and Vance, J. E.) Mento Park, CA: Benjamin-CummingsGoogle Scholar
Sears, J. K. & Darby, J. R. 1982 The Technology of Plasticizers. New York: Wiley InterscienceGoogle Scholar