Hostname: page-component-848d4c4894-nr4z6 Total loading time: 0 Render date: 2024-04-30T12:50:24.124Z Has data issue: false hasContentIssue false
  • English
  • Français

Two mutations giving low-level streptomycin resistance in Escherichia coli K12

Published online by Cambridge University Press:  14 April 2009

Lilian M. Roberts  and
E. C. R. Reeve
Lilian M. Roberts
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh 9
E. C. R. Reeve
Affiliation:
Institute of Animal Genetics, West Mains Road, Edinburgh 9
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A mutation in Escherichia coli K12 giving resistance to about 5 μg/ml of streptomycin was found to be cotransducible by P1 with proA and proB, and is located at about 8·5 min on the chromosome map. The locus is named strB. A second mutation to the same resistance level was not cotransducible with either proA or proB and must be located elsewhere. Both mutations cause a marked increase in R-factor mediated streptomycin resistance, and significant decreases in resistance to several other antibiotics, both in the presence and absence of an R-factor determinant for the same antibiotic. The two mutations differ in their effects on bacterial sensitivity to crystal violet and EDTA.

Type
Short Papers
Information
Genetics Research , Volume 16 , Issue 3 , December 1970 , pp. 359 - 365
Copyright
Copyright © Cambridge University Press 1970

References

REFERENCES

Bryson, V. & Demerec, M. (1950). Patterns of resistance to antimicrobial agents. Annals of the New York Academy of Sciences 53, 283–289.CrossRefGoogle ScholarPubMed
Clowes, R. C. & Hayes, W. (ed.) (1968). Experiments in Microbial Genetics. Oxford: Blackwell.Google Scholar
Curtiss, R. III (1965). Chromosomal aberrations associated with mutations to bacteriophage resistance in Escherichia coli. Journal of Bacteriology 89, 28–40.CrossRefGoogle Scholar
Demerec, M. (1948). Origin of bacterial resistance to antibiotics. Journal of Bacteriology 56, 63–74.CrossRefGoogle ScholarPubMed
Low, B. (1965). Low recombination frequency for markers very near the origin in conjugation in E. coli. Genetical Research 6, 469–473.CrossRefGoogle ScholarPubMed
Markovitz, A. (1964). Regulatory mechanisms for synthesis of capsular polysaccharides in Escherichia coli K12. Proceedings of the National Academy of Sciences of the United States of America 51, 239–246.CrossRefGoogle ScholarPubMed
Nordström, K., Burman, L. G. & Eriksson-Grennberg, K. G. (1970). Resistance of Escherichia coli to penicillins, VIII. Physiology of a class II ampicillin-resistant mutant. Journal of Bacteriology 101, 659–668.CrossRefGoogle Scholar
Normark, S., Boman, H. G. & Matsson, E. (1969). A mutant of Escherichia coli K12 with anomalous cell division and ability to decrease episomally and ehromosomally mediated resistance to ampicillin and several other antibiotics. Journal of Bacteriology 97, 1334–1342.CrossRefGoogle Scholar
Pearce, L. E. & Meynell, E. (1968). Mutation to high level streptomycin resistance in R+ bacteria. Journal of General Microbiology 50, 173–176.CrossRefGoogle ScholarPubMed
Reeve, E. C. R. (1966). Characteristics of some single-step mutants to chloramphenicol resistance in E. coli K12 and their interactions with R-factor genes. Genetical Research 7, 281–286.CrossRefGoogle Scholar
Taylor, A. L. (1970). Current linkage map of Escherichia coli. Bacteriological Reviews 34, 155–175.CrossRefGoogle ScholarPubMed
Taylor, A. L. & Trotter, C. D. (1967). Revised linkage map of Escherichia coli. Bacteriological Reviews 31, 332–353.CrossRefGoogle ScholarPubMed
Vogel, H. J. & Bonner, D. M. (1956). Acetylornithinase of Escherichia coli: partial purification and some properties. Journal of Biological Chemistry 218, 97–106.CrossRefGoogle ScholarPubMed
Watanabe, T. & Watanabe, M. (1959). Transduction of streptomycin sensitivity into resistant mutant of Salmonella typhimurium. Journal of General Microbiology 21, 30–39.CrossRefGoogle ScholarPubMed