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Evaluation of antioxidant potential of leaves of Leonotis nepetifolia and its inhibitory effect on MCF7 and Hep2 cancer cell lines

https://doi.org/10.1016/S2222-1808(13)60053-5Get rights and content

Abstract

Objective

To investigate the antioxidant and antiproliferative potential of Leonotis nepetifolia (L. nepetifolia) leaves.

Methods

The leaves of L. nepetifolia were subjected to extraction using three different solvents and the antioxidant potential of those extracts were tested by using various in vitro assays. Further, the best screened extract was analyzed for its phytochemical profile by both qualitative and quantitative assays. In order to determine its anti-proliferative activity, the best screened extract was treated with breast and laryngeal cancer cell lines such as MCF-7 cells and Hep2 cells, respectively. The cytotoxicity of the extract was also studied using MTT assay. The inhibitory effect of the extract of leaves of L. nepetifolia on the selected cell-line DNA was determined by DNA fragmentation assay. Also, the extract was subjected to TLC and bioautography analysis.

Results

The DPPH assay showed methanol extract of L. nepetifolia leaves to be more significant in scavenging free radicals with inhibition percentage of 60.57%. From the data obtained, the methanol extract proved to be significant in all anti-oxidant assays and this effect was well comparable with the standard used in the study. The predominant phytochemicals such as phenols and flavonoids were further quantified as 0.107% and 0.089%. The cytotoxicity assay showed that the cell viability increased with increasing concentration of methanol extract. In addition, the extract caused dose dependent damage to the cancer cell lines MCF-7 and Hep2.

Conclusions

Our study suggests that the leaves of L. nepetifolia were significant in scavenging free radicals and causing damage to proliferative cells. Further mechanistic studies would help in proving the efficiency of the selected plant under in vivo conditions.

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  • Cited by (0)

    Available online 28 Apr 2013

    Peer reviewer Dr. M. Jayaprakashvel, Assistant Professor, Department of Biotechnology, AMET University (u/s3 of UGC Act 1956), 135, ECR, Kanathur, Chennai-603112, India., Tel: +91 9840529274, E-mail: [email protected]

    Comments This is a good study and considered for publication in the journal. Because, it is a very basic study, it has very good scope to further continue this work with more advanced tools to prove their anticancer activity.

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