Skip to main content
SpringerLink
Log in

Using a Surface Plasmon Resonance Biosensor for Rapid Detection of Salmonella Typhimurium in Chicken Carcass

  • Published:
Journal of Bionic Engineering Aims and scope Submit manuscript

Abstract

Chicken is one of the most popular meat products in the world. Salmonella Typhimurium is a common foodborne pathogens associated with the processing of poultry. An optical Surface Plasmon Resonance (SPR) biosensor was sensitive to the presence of Salmonella Typhimurium in chicken carcass. The Spreeta biosensor kits were used to detect Salmonella Typhimurium on chicken carcass successfully. A taste sensor like electronic tongue or biosensors was used to basically “taste” the object and differentiated one object from the other with different taste sensor signatures. The surface plasmon resonance biosensor has potential for use in rapid, real-time detection and identification of bacteria, and to study the interaction of organisms with different antisera or other molecular species. The selectivity of the SPR biosensor was assayed using a series of antibody concentrations and dilution series of the organism. The SPR biosensor showed promising to detect the existence of Salmonella Typhimurium at 1 × 106 CFU/ml. Initial results show that the SPR biosensor has the potential for its application in pathogenic bacteria monitoring. However, more tests need to be done to confirm the detection limitation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Green D K. Sterilization Apparatus, US Patent No. 4457221, 1987.

    Google Scholar 

  2. Food Safety: The Agricultural Use of Antibiotics and its Implications for Human Health, Letter Report, GAO/RCED-99-74, USDA, 1999.

  3. Seo K H, Brackett R E, Hartman N F, Campbell D P. Development of a rapid response biosensor for detection of Salmonella Typhimurium. Journal of Food Protection, 1999, 62, 431–437.

    Article  Google Scholar 

  4. Fratamico P M, Strobaugh T P, Medina M B, Gehring A G. Detection of Escherichia Coli O157:H7 using a surface plasmon resonance biosensor. Biotechnology Techniques, 1998, 12, 571–576.

    Article  Google Scholar 

  5. Elkind J L, Stimpson D I, Strong A, Bartholomew D U, Melendez J L. Integrated analytical sensors: The use of the TISPR-1 as a biosensor. Sensor and Actuators B:Chemical, 1999, 54, 182–190.

    Article  Google Scholar 

  6. Buzby J C, Roberts T. Microbial foodborne illness. Choices, First Quarter, 1996, 14–17.

    Google Scholar 

  7. Buzby J, Roberts T, Liu C T, MacDonald J. Bacterial Foodborne Disease: Medical Costs and Productivity Losses, USDA Agricultural Rural Report, No. 741, 1996.

    Google Scholar 

  8. Lillard H S. The impact of commercial processing procedures on bacterial contamination and cross-contamination of broiler carcases. Journal of Food Protection, 1990, 53, 202–204.

    Article  Google Scholar 

  9. Center for Food Safety and Applied Nutrition, [2008-05-06], http://vm.cfsan.fda.gov

  10. Meeusen C. Evaluation of a Surface Plasmon Resonance Biosensor for the Identification of Salmonella Typhimurium and Escherichia Coli O157:H7 in the Pork Production Chain, M.Sc. Thesis, Michigan State University, East Lasing, MI, 2000.

    Google Scholar 

  11. Moutville T J. Food Microbiology, CRC Press, NY, 1987.

    Google Scholar 

  12. Jay J M. Modern Food Microbiology, Sixth Edition, Aspen Publishers, Inc. Gaithersburg, MD, 2000.

    Book  Google Scholar 

  13. Deshpande S S. Immunodiagnostics in agricultural, food, and environmental quality control. Food Technology, 1994, 48, 136–141.

    Google Scholar 

  14. Turner A P, Newman J D. An Introduction to Biosensors, Biosensors for Food Analysis, Scott A O (ed), Athenaeum Press Ltd, Gateshead, UK, 1998.

  15. Jonsson U. Optical Affinity Biosensors, Biosensors for Food Analysis, Scott A O (ed.) Athenaeum Press Ltd, Gateshead, UK, 1998.

  16. Operation Manual, Spreeta Experimenter’s Kit, Texas Instruments, Inc. Dallas, Texas, USA, 1999.

  17. Salamon Z, Brown M F, Tollin G. Plasmon resonance spectroscopy: Probing molecular interactions within membranes. Trends in Biochemical Sciences, 1999, 24, 213–219.

    Article  Google Scholar 

  18. Cranfield Biotechnology Center, [2008-04-21], http://www.cranfieldac.uk/biotech/spr.htm

  19. Demarco D, Saaski E, McCrae D, Lim D. Rapid detection of Escherichia coli O157:H7 in ground beef using a fiber optic biosensor. Journal of Food Protection, 1999, 62, 711–716.

    Article  Google Scholar 

  20. Meeusen C, Alocilja E, Osburn W. Detection of pathogenic bacteria in the pork industry chain using a surface plasmon resonance biosensor. Part 1: Sensor verification. 93rd Annual International Meeting of ASAE, Milwaukee, Wisconsin, USA, 2000, paper no. 006046.

    Google Scholar 

  21. Meeusen C, Alocilja E, Osburn W. Evaluation of a surface plasmon resonance biosensor in the detection of Salmonella spp. ASAE, St. Joseph, MI, 2001a, paper no. 016035.

    Google Scholar 

  22. Meeusen C, Alocilja E Osburn, Ryser E. Use of biosensor for pathogen monitoring in the pork production chain. ASAE, St. Joseph, MI, 2001b, paper no. 017031.

    Google Scholar 

  23. Meeusen C, Alocilja E, Osburn W. Detection of E. coli O157:H7 using a surface plasmon resonance biosensor. ASAE, St. Joseph, MI, 2001c, paper no. 017030.

    Google Scholar 

  24. Wortberg M, Orban M, Renneberg R, Cammann K. Fluorimetric immunosensors. In Kress-Rogers (ed.), Handbook of Biosensors and Electronic Noses, CRC, Boca Raton, Florida, 1997.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. USDA-ARS, College Station, TX, 77845, USA

    Yu-bin Lan & W. Clint Hoffmann

  2. Department of Environmental and Occupational Health, Texas A&M University, College Station, TX, 77845, USA

    Shi-zhou Wang

  3. College of Biological and Agricultural Engineering, Jilin University, Changchun, 130022, P. R. China

    Yong-guang Yin

  4. College of Engineering, Northeast Agricultural University, Harbin, 150030, P. R. China

    Xian-zhe Zheng

Authors
  1. Yu-bin Lan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shi-zhou Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yong-guang Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. W. Clint Hoffmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xian-zhe Zheng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yu-bin Lan.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lan, Yb., Wang, Sz., Yin, Yg. et al. Using a Surface Plasmon Resonance Biosensor for Rapid Detection of Salmonella Typhimurium in Chicken Carcass. J Bionic Eng 5, 239–246 (2008). https://doi.org/10.1016/S1672-6529(08)60030-X

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1016/S1672-6529(08)60030-X

Keywords

Search

Navigation