Fluorescence-activated cell sorting of transfected cells
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Systematic and quantitative analysis of G protein-coupled receptor trafficking motifs
2013, Methods in EnzymologyCitation Excerpt :A proper FACS analysis requires measurements of nontransfected cells and determination of cell death to ensure accurate measurements of relative surface and total cell receptor levels. A more complete description of FACS analysis of transfected cells can be found elsewhere (Adams, Lopez, Sellers, & Kaelin, 1997). Split a 10-cm tissue culture dish of confluent HEK293 cells 1:10 in DMEM with 10% FBS.
Hydrodynamic gating valve for microfluidic fluorescence-activated cell sorting
2010, Analytica Chimica ActaCitation Excerpt :However, it usually requires a population of cells more than 100,000 in order to achieve a high yield, which limits its applications in small-scale cell sorting such as isolation of rare cells. For example, transient gene transfection of cells in a 96-well dish usually yields less than 100,000 cells with a 5–30% efficiency [4]. The resulting cells need to be purified before further studies of gene function and expression can be conducted.
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