Direct detection of paramagnetic species in adriamycin perfused rat hearts

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Direct detection of paramagnetic species in control and adriamycin-perfused rat hearts has been carried out. Depending on the flow rate of the perfusion solution(8,4,2 and 1 ml/min) different paramagnetic species were observed: Fe(III)(g=2.12)at 4 ml/min; three types of oxygen centered radicals of which two in control hearts(g1=2.05 g2=2.038 g1=g2=2.008) and the third one (g=2.03 g=2.005) in adriamycin perfused hearts, at 2 ml/min. The latter radical was the only one observed at perfusion rate of 1 ml/min both in control and adriamycin treated systems. A relationship between the intracellular enzymatic reductive activation of the anthracycline and the occurrence of ischemic conditions(4,2 and 1 ml/min) in myocardial tissues is proposed basing on the relative amounts of the paramagnetic species above mentioned.

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