Lacking applicability of in vitro eye irritation methods to identify seriously eye irritating agrochemical formulations: Results of bovine cornea opacity and permeability assay, isolated chicken eye test and the EpiOcular™ ET-50 method to classify according to UN GHS
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Keywords
Serious eye damage
EpiOcular ET-50
Isolated chicken eye test
(Modified) bovine corneal opacity and permeability test
Agrochemical formulation
Classification
Abbreviations
AAALAC
Association for Assessment and Accreditation of Laboratory Animal Care
BCOP
bovine corneal opacity and permeability
Cat
Category
CC
conjunctival chemosis
CD
conjunctival discharge
CO
corneal opacity
CR
conjunctival redness
EIT
eye irritation test
ET-50
exposure time reducing viability of treated tissue to 50%
EURL ECVAM
European Reference Laboratory European Centre for the Validation of Alternative Methods
HET-CAM
Hen's Egg Test- Chorioallantoic Membrane
HSI
histopathological score of irritation
IATA
integrated approaches for testing and assessment
ICE
Isolated Chicken Eye
II
iris inflammation
IVIS
in vitro irritation score
NC
negative control
OD
optical density
NI
non-irritant (not classified according to UN GHS)
NP
no prediction can be made
OECD
Organization for Economic Co-operation and Development
P
present
SD
standard deviation
TG
test guideline
UN GHS
United Nations Globally Harmonized System
1a
effects observed in one animal
2a
effects observed in two animals
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© 2017 The Authors. Published by Elsevier Inc.