Elsevier

Genomics

Volume 112, Issue 1, January 2020, Pages 930-933
Genomics

De novo assembly and annotation of the Ganoderma australe genome

https://doi.org/10.1016/j.ygeno.2019.06.008Get rights and content
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Highlights

  • De novo sequencing of the Ganoderma australe genome, including assembly and

  • gene predictions for the 84 megabase genome.

  • Prediction of 22,756 protein-coding genes using the funannotate pipeline.

  • Prediction of five putative genes and two enzyme complexes from a ganoderic acid pathway within the G. australe genome.

Abstract

The Ganoderma genus represents clear biotechnological potential, due to the large quantity of molecules with biological activity that could be explored. However, available information regarding the biotechnological importance of species within Ganoderma, other than G. lucidum, is quite limited. Genomic studies of little-known species can contribute to the knowledge thereof, as well as the search for metabolic pathways and the identification of genes which code for proteins that may be of biotechnological relevance. Therefore, the objective of the present study was to obtain the G. australe genome, through the use of new sequencing technologies. Genomic DNA from G. australe was sequenced with the PacBio Sequel system, to a depth of 100×. The genome was assembled de novo with the Canu assembly tool, and gene prediction and annotation were performed with a funannotate pipeline. An assembled 84 Mb genome was obtained, and 22,756 putative protein-coding sequences were predicted in the G. australe genome. Ganoderic acid pathways were annotated and listed in the funannotate pipeline, and were recognized using Pfam and Antismash signals. Thus, the G. australe genome shows great potential, mainly, due to the annotation of putative sequences that could be employed in biotechnological approaches.

Keywords

Bioinformatics
Assembly
Ganoderma
Genome

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