Co-culture of primary mouse neurons and microglia for high-throughput assays
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Model neurodegeneration in the spinal cord with oxidized phosphatidylcholine injection
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In vitro and in vivo approaches to study roles of microglia during neurodegeneration
Summary
Oxidized phosphatidylcholine (OxPC) found in multiple sclerosis brain lesions mediates neurodegeneration. Microglia are prominent responders to the OxPC insult, and thus, studying their protective or noxious functions is important to help halt neurodegeneration. Here, we present protocols including cell isolation and culture, animal surgeries, as well as tissue processing and isolation to study the microglia response to OxPC-mediated neurodegeneration in vitro and in vivo.
For complete details on the use and execution of this protocol, please refer to Dong et al. (2021).
Graphical abstract
Subject areas
Cell culture
Cell isolation
Cell-based Assays
Immunology
Microscopy
Model Organisms
Neuroscience
Data and code availability
The published article includes all datasets generated or analyzed during this study.