STAR Protocols
Volume 2, Issue 4, 17 December 2021, 100853
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Protocol
Studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord

https://doi.org/10.1016/j.xpro.2021.100853Get rights and content
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Highlights

  • Co-culture of primary mouse neurons and microglia for high-throughput assays

  • Model neurodegeneration in the spinal cord with oxidized phosphatidylcholine injection

  • In vitro and in vivo approaches to study roles of microglia during neurodegeneration

Summary

Oxidized phosphatidylcholine (OxPC) found in multiple sclerosis brain lesions mediates neurodegeneration. Microglia are prominent responders to the OxPC insult, and thus, studying their protective or noxious functions is important to help halt neurodegeneration. Here, we present protocols including cell isolation and culture, animal surgeries, as well as tissue processing and isolation to study the microglia response to OxPC-mediated neurodegeneration in vitro and in vivo.

For complete details on the use and execution of this protocol, please refer to Dong et al. (2021).

Subject areas

Cell culture
Cell isolation
Cell-based Assays
Immunology
Microscopy
Model Organisms
Neuroscience

Data and code availability

The published article includes all datasets generated or analyzed during this study.

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