Elsevier

Virology

Volumes 464–465, September 2014, Pages 206-212
Virology

Histochemical fluorescent staining of Sendai virus-infected cells with a novel sialidase substrate

https://doi.org/10.1016/j.virol.2014.04.005Get rights and content
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Highlights

  • We develop a novel sialidase substrate that can stain Sendai virus-infected cells.

  • The substrate can perform histochemical fluorescent staining of the infected cells.

  • The substrate can easily, rapidly, and sensitively visualise the infected cells.

  • The substrate can also visualise infected tissues of mice inoculated with the virus.

  • The substrate will contribute to further virological study in the future.

Abstract

Sialidases, enzymes that remove terminal sialic acid residues, are pivotal in various biological processes such as malignancy and infection with pathogens. For histochemical staining of sialidase activity, we have developed a new synthetic sialidase substrate, sialic acid-conjugated fluorescent benzothiazolylphenol derivative (BTP3-Neu5Ac), for rapid, sensitive, and specific fluorescent staining of sialidase activity. Here, we showed the usefulness of BTP3-Neu5Ac for histochemical fluorescent staining of cells infected with Sendai virus (SV), which possesses sialidase activity. BTP3-Neu5Ac also visualised SV-infected regions of lung sections from SV-infected mice. We succeeded in histochemical fluorescent staining of SV both in vitro and in vivo. SV has been utilised in many virological and biotechnological studies such as developments of an oncolytic virus, a gene therapy vector, and a vaccine candidate. BTP3-Neu5Ac should contribute to rapid progress of such studies and researches on viral sialidase.

Keywords

Sialidase activity
Sendai virus
Histochemical staining
Fluorescent staining
Hemagglutinin-neuraminidase
Synthetic substrate
Infection detection
Easy protocol
Rapid protocol
Sensitive detection

Cited by (0)

1

They contributed equally as first authors.