Optimal Conditions for Oxygenated Subnormothermic Machine Perfusion for Liver Grafts Using a Novel Perfusion Device
Section snippets
Material and Methods
All experiments were conducted in accordance with the Guide for the Care and Use of Laboratory Animals prepared by the National Academy of Sciences and published by the National Institute of Health [17] and the guidelines for animal experiments at Tohoku University. This study was approved by the institutional animal care committee of Tohoku University (2019MdA-045-04).
Bile Production
Bile production in the HB group (30 minutes: 17.87 ± 9.65 μL/kg; 60 minutes: 22.4 ± 28.5 μL//kg; 90 minutes: 29.76 ± 25.24 μL/kg; 120 minutes: 26.34 ± 18.74 μL/kg) was significantly higher than that in the DCD group (30 minutes: 4.91 ± 9.65 μL/kg; 60 minutes: 25.08 ± 36.03 μL//kg; 90 minutes: 33.37 ± 53.15 μL/kg; 120 minutes: 27.95 ± 34.38 μL/kg) at 30 minutes after the start of perfusion (P = .043), but no significant differences were found at other time points (Fig 3).
AST, ALT, LDH, and IL-1β Levels in the Perfusate
AST (30 minutes: 399.0 ±
Discussion
In this study, we investigated the optimal perfusion conditions for DCD grafts using a new perfusion device because the optimal perfusion conditions for organ perfusion have not yet been determined in the literature. To investigate the optimal duration of oxygenated subnormothermic perfusion, we performed 120-minute perfusions and examined the condition of the organ and release of waste products into the reflux fluid during the perfusion. The results showed that the release of liver enzymes
Conclusion
In this study, we investigated the optimal time for MP of DCD grafts using a novel perfusion device. In the future, accumulation of more cases is necessary to confirm the safety of this new perfusion device and expand the indications for DCD liver transplantation.
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2023, Chinese Journal of Hepatobiliary Surgery