Ischemia/reperfusion injuryExperimental: IntestinalCurcumin Nutrition for the Prevention of Mesenteric Ischemia–Reperfusion Injury: An Experimental Rodent Model
Section snippets
Experimental Design
Adult male Wistar albino rats weighing between 170 and 230 g were obtained from the Istanbul University DETAM Institute and kept at room temperature (20–22°C) with exposure to 12 hour cycles of light/dark. The rats were fed standard how and tap water. Ethical consent was obtained from our Ethics Committee and procedures conducted in accordance with the Guide for the Care and Use of Laboratory Animals by the National Institutes of Health (NIH publication no. 85-23, revised 1996).
Thirty rats were
Results
The changes in hemoglobin and hematocrit after I/R injury did not show any significant differences among the 3 groups. Furthermore, there were no significant intergroup differences in other biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), lactate, blood urine nitrogen, and creatinine.
MPO values were 1,150 ± 0.27 in group I; 1,500 ± 0.35 in group II and 1,300 ± 0.30 in group III (Fig 1). The differences between the
Discussion
Hypoxia and reoxygenation are generally considered to be the main factors leading to tissue damage during I/R injury. Many studies have demonstrated that the major component of the adverse reaction is not initiated by hypoxia but, rather, by the return of the oxygenated blood to previously ischemic tissue.10, 11 Several endogenous substances, including oxygen free radicals, platelet activating factor, arachidonic acid metabolites, and bacterial endotoxins have been implicated in the
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2013, PhytomedicineCitation Excerpt :In the present study, we hypothesized that curcumin may have a protective role in intestinal IR induced remote organ injury due to its antiinflammatory and antioxidant effects. To the best of our knowledge, only two studies (Karatepe et al. 2009; Yucel et al. 2011) have been investigated the effect of curcumin supplementation in intestinal IR. Additionally in a recent study, Guzel et al. (2012) have been demonstrated that short term curcumin supplementation (3 days) has protective effect on intestinal IR induced acute lung injury.
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Curcumin suppresses the Wnt/β-catenin signaling pathway by inhibiting NKD2 methylation to ameliorate intestinal ischemia/reperfusion injury
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