Pancreas and islet transplantationSmall Intestinal Submucosa Improves Islet Survival and Function in Vitro Culture
Section snippets
Animals
Male Wistar rats (250 to 400 g body weight, Animal Lab of Xi’an Jiaotong University, China) were used for islet isolation. All animals received care in compliance with the guidelines of the local Animal Care and Use Committee following National Institutes of Health guidelines.
Design of the Study
Islets were isolated by using collagenase and purified by discontinuous Euroficoll gradients. The purified islets were separated into two experimental groups: the study group were islets cultured in plates coated with SIS,
Results
After 7 or 14 days of in vitro tissue culture, the group of islets cultured with SIS showed a twofold greater (P < .05) recovery than the control group (Table 1). In the SIS-treated group, there was no statistical difference between the short and the long periods of culture (95.8% ± 1.0% vs 90.8% ± 1.5%, P > .05). Most islets cultured in the control group lost their initial morphology, becoming “loose” in appearance at the end of the culture, where as islets cultured with SIS exhibited
Discussion
Islet transplantation is now a viable option for the treatment of insulin-dependent diabetes mellitus. Maintaining islets in culture is important to increase the safety, practicality, and efficacy imperative for successful clinical results. In addition to reducing the immunogenicity of islets,3, 17 culture simplifies the logistics of transplantation—the procedure can be scheduled during regular hospital hours, and the patient does not have to move from a distant location to the transplantation
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2009, Transplantation ProceedingsCitation Excerpt :The laser beam irradiated the wells from a distance of 5-mm. To evaluate the in vitro function of the islets after laser irradiation, they were placed in 2 glucose concentrations, 2.8 mM basal and 16.7 mM glucose, then incubated at 37°C for 30 minutes.9 Insulin concentrations were assessed in buffer media using a rat insulin enzyme-linked immunosorbent assay kit (Mercodia, Sweden) according to the manufacturer's protocol.
This work was supported by the Key Program of Science & Technique of Ministry of Education of the People’s Republic of China (No. 104169).