Elsevier

Stem Cell Research

Volume 57, December 2021, 102578
Stem Cell Research

Lab Resource: Genetically-Modified Single Cell Line
Generation of a TLR2 knockout human induced pluripotent stem cell line using CRISPR/Cas9

https://doi.org/10.1016/j.scr.2021.102578Get rights and content
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Abstract

The TLR family plays a fundamental function in the recognition of pathogens and activation of innate immunity. TLR2 is a membrane protein that is expressed on the surface of certain cells and recognizes foreign substances; it initiates a cascade of innate immune responses. TLR2 recognizes many bacterial, fungal, and viral components, as well as certain endogenous substances. We generated biallelic mutants of the TLR2 gene in human induced pluripotent stem cells (hiPSCs) using a CRISPR-Cas9 genome editing method. The TLR2 heterozygous-knockout hiPSCs retained normal morphology, gene expression, and in vivo differentiation potential.

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