Elsevier

Stem Cell Research

Volume 51, March 2021, 102204
Stem Cell Research

Lab Resource: Multiple Cell Lines
Generation of six human induced pluripotent stem cell sublines (MZT01E, MZT01F, MZT01N and MZT02D, MZT02G and MZT02H) for reproductive science research

https://doi.org/10.1016/j.scr.2021.102204Get rights and content
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Highlights

  • Six independent hiPSC sublines from twin sisters discordant for ovarian failure.

  • The hiPSC sublines are equivalent in measures of pluripotency and self-renewal.

  • These lines are consented for research in the reproductive sciences.

Abstract

Six human induced pluripotent stem cell sublines (hiPSCs) were generated from human dermal fibroblasts (HDFs) derived from skin biopsies donated from monozygotic twin women wherein one woman had proven fertility and her sister was infertile due to ovarian failure. Three hiPSC sublines were created from each twin’s HDFs. hiPSCs were reprogrammed using Sendai virus vectors and were subsequently positive for markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA-4. Pluripotency was further verified using PluriTest. We show here that the hiPSC lines created from the twins are equivalent in measures of pluripotency and self-renewal, despite their differential diagnosis.

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