Regular ArticleDirected differentiation of porcine epiblast-derived neural progenitor cells into neurons and glia
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Highlights
►Three porcine neural progenitor cell (NPC) lines were derived from epiblast cells. ► They all express the markers SOX2, NESTIN, VIMENTIN and KI67. ► One detailed analyzed NPC line could be differentiated into neural and glial subtypes. ► The NPCs may be used in future transplantation experiments in large animal models.
Abbreviations
AA
Ascorbic acid
BDNF
Brain-derived neurotrophic factor
bFGF
Basic fibroblast growth factor
ESC
Embryonic stem cell
FGF8
Fibroblast growth factor 8
GDNF
Glial cell line-derived neurotrophic factor
ICM
Inner cell mass
iPSC
Induced pluripotent stem cell
LIF
Leukemia inhibitory factor
MEF
Mouse embryonic fibroblast
MPTP
1 - methyl 4 - phenyl - 1, 2, 3, 6 - tetrahydropyridine
MS5
Mouse stromal cell line 5
MZ
Marginal zone
NPC
Neural progenitor cell
OC
Outgrowth colony
PDGF
Platelet-derived growth factor
RA
Retinoic Acid
SHH
Sonic Hedgehog
SGZ
Subgranular zone
SZ
Subventricular zone
VZ
Ventricular zone
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