Spatial analysis and source profiling of beta-agonists and sulfonamides in Langat River basin, Malaysia
Graphical abstract
Introduction
Beta-agonists and sulfonamides are among the most commonly used veterinary drugs worldwide. Beta-agonists are used to treat cardiogenic shock, acute heart failure, bradyarrhythmias, asthma and chronic obstructive pulmonary disease (Yu et al., 2011), and also to feed them as a growth promoter in livestock feed for considerable muscle mass increase while decreasing fat accumulation (Mersmann, 1998). Similarly, sulfonamides are widely used in livestock productions for their therapeutic and prophylactic properties (Malintan & Mohd, 2006) as well as for growth promotion (Cheong et al., 2010). Environmental pollution of these drugs is concerned and beta-agonists may lead to adverse cardiovascular events (Salpeter et al., 2004), while sulfonamides may cause drug-resistant bacteria (Huovinen et al., 1995). Furthermore, beta-agonists and sulfonamides are used to treat diseases and infections for human, and they had been detected in waste streams from wastewater treatment plants, sewage treatment plants, hospitals and drug production facilities (Yu et al., 2011). Therefore, there is the potential for contamination of the water by such a wide range of treatments for both human and livestock. In Malaysia, the potential usage of banned beta-agonists in swine farms was identified (Ponniah et al., 2004), and salbutamol was detected in effluents of sewage treatment (Al-Odaini et al., 2010 and Al-Odaini et al., 2013). Likewise, sulfonamides were detected in effluents from swine farms (Malintan & Mohd, 2006), and the residues were detected in chicken meat products (Cheong et al., 2010). Since sulfonamides have high potentials to resist degradation and are highly mobile (Luo et al., 2011), they could be extensively transported from pollution sources flowing surface water.
The Langat River basin is located in the southern part of the capital region (i.e. Klang Valley) in Peninsular Malaysia, and has a major role in regional water supply. Urbanization in the Langat River basin drastically progressed due to the acceleration of economic growth since the 1980s. As a consequence, surface runoff had increased with a positive relationship with urban-related land uses (Noorazuan et al., 2003). Moreover, water quality in the Langat River basin has deteriorated due to wastewater from industry and households, municipal waste and agricultural runoff (Juahir et al., 2011). Drinking water treatment facilities have been frequently shut down due to a high concentration of ammonia in intake water over the last two decades (Hasan et al., 2010).
According to our preliminary survey, surface water was heavily contaminated by Escherichia coli (E. coli) around urban sites and E. coli were detected even in upstream regions. It is probably because household coverage by piped sewers is inadequate and septic tanks are poorly maintained. Furthermore, there is no specific regulation or federal law for the livestock effluent standards except for the regulations that were enforced through various state enactments and by-laws. Therefore, farmers usually treat their wastewater by sedimentation ponds or any biological waste treatment systems. In addition, some livestock species such as cattle, dairy cow and goat were sometimes released to graze outside. Both insufficiently treated livestock effluents and the excrement left in grazing fields could be transported into rivers. Thus, these various point sources and non-point sources would result in the severe contamination of E. coli.
Likewise, severe environmental pollution of beta-agonists and sulfonamides is concerned in the Langat River basin. There are populated cities and towns located in mid catchment areas and a large scale of oil palm plantation and livestock farms around the southern part of the mid catchment areas and entire downstream areas. The variety of anthropogenic influence could cause the contamination of these drugs and make the pollution complicated to identify the sources by environmental monitoring. Therefore, a spatial analysis and a source profiling are also necessary to visualize the occurrence and distribution as well as to identify the pollution sources.
Distribution of contaminants in river basin is basically followed by elevation and flow direction of surface water in a gravitational way, which means that occurrence of contaminants detected in a sampling point is subject to its catchment area. The pollutant load could become proportional to a number of pollution sources (i.e. point source) and/or an area of pollution sources (i.e. non-point source) in the catchment area. As long as there is no significant regional difference of coverage of sewage treatment system and hydrological impacts like abstraction of river water among catchment areas, the pollutant load in surface water would have a correlation with the number and/or the area of pollution sources. If that is the case, the pollutant loads of beta-agonists and sulfonamides could have a correlation with human and/or livestock population, considering their usage for treatments. Therefore, this study aimed to analyze these drugs throughout the Langat River basin for clarifying the contamination, and also to conduct the spatial analysis and source profiling for identifying the pollution source by using geographic information system (GIS).
Section snippets
Standards and reagents
Fourteen beta-agonist standards (cimaterol, terbutaline, salbutamol, zilpaterol, cimabuterol, ractopamine, clenbuterol, brombuterol, tulobuterol, mabuterol, hydroxymethyl clenbuterol, clenpenterol, isoxsuprine and mapenterol), 2 beta-agonist surrogates (ractopamine-d3 and clenbuterol-d9) and 12 sulfonamide standards (sulfadiazine, sulfathiazole, sulfapyridine, sulfamerazine, sulfamethazine, sulfamethizole, sulfamethoxypyridazine, sulfadimethoxine, sulfaquinoxaline, sulfamethoxazole,
Water quality monitoring and assessment
Table 2 shows in-situ water quality monitoring results in 14 sampling sites. The pH was neutral in S4–S14 (6.28–7.26), whereas it was acid in S1, S2 and S3 (4.47–5.72) probably because of peatland around these points. The peatland exists at downstream areas of the Langat River basin and the low pH occurs because of acid sulfate soils (Ng et al., 1994 and Wöstena et al., 1997). The peatland had been extensively developed for oil palm plantation and it caused subsidence and oxidation of pyrite (
Conclusions
This study detected 6 compounds of beta-agonists and sulfonamides in the surface water collected in the Langat River basin. It was suggested that the main pollution source of salbutamol and sulfamethoxazole was from sewage, while sulfadiazine was discharged from effluents of cattle, goat and sheep farms. The source profiling did not work for sulfadimethoxine, sulfapyridine and sulfamethazine, but the areas of the pollution sources were identified by the spatial analysis. These drugs were
Acknowledgment
This study was funded by UM/MOE HIR Grant (E000005-20001). Also, this work was supported under the framework of “Research and Education Center for Risk Based Asian Oriented Integrated Watershed Management,” funded by the JSPS Asian CORE Program and Ministry of Education, Malaysia.
References (28)
- et al.
Multi-residue analytical method for human pharmaceuticals and synthetic hormones in river water and sewage effluents by solid-phase extraction and liquid chromatography–tandem mass spectrometry
J. Chromatogr. A
(2010) - et al.
Occurrence of sulfonamide residues along the Ebro river basin: removal in wastewater treatment plants and environmental impact assessment
Environ. Int.
(2011) - et al.
Effect of chromatographic conditions on liquid chromatographic chiral separation of terbutaline and salbutamol on Chirobiotic V column
J. Chromatogr. A
(2011) - et al.
Fate of sulfadiazine administered to pigs and its quantitative effect on the dynamics of bacterial resistance genes in manure and manured soil
Soil Biol. Biochem.
(2008) - et al.
The occurrence and removal of selected pharmaceutical compounds in a sewage treatment works utilising activated sludge treatment
Environ. Pollut.
(2007) - et al.
Effect of organic carbon and pH on soil sorption of sulfamethazine
Chemosphere
(2009) - et al.
Determination of sulfonamides in selected Malaysian swine wastewater by high-performance liquid chromatography
J. Chromatogr. A
(2006) - et al.
A preliminary study on the occurrence and behavior of sulfonamides, ofloxacin and chloramphenicol antimicrobials in wastewaters of two sewage treatment plants in Guangzhou, China
Sci. Total Environ.
(2006) - et al.
Cardiovascular effects of beta-agonists in patients with asthma and COPD: a meta-analysis
Chest
(2004) - et al.
The occurrence of human pharmaceuticals in wastewater effluents and surface water of Langat River and its tributaries, Malaysia
Int. J. Environ. Anal. Chem.
(2013)
Sulfonamides determination in chicken meat products from Malaysia
Int. Food Res. J.
Problems of ammonia and manganese in Malaysian drinking water treatments
World Appl. Sci. J.
Trimethoprim and sulfonamide resistance
Antimicrob. Agents Chemother.
Country sanitation assessment in Malaysia report
Cited by (37)
Localized pollution of veterinary antibiotics in watersheds receiving treated effluents from swine farms
2023, Science of the Total EnvironmentApplication of wastewater-based epidemiology to estimate the usage of beta-agonists in 31 cities in China
2023, Science of the Total EnvironmentOccurrence of endocrine disruptors in Malaysia's water systems: A scoping review
2023, Environmental PollutionSpatiotemporal distribution of veterinary and human drugs and its predictability in Japanese catchments
2023, Science of the Total Environment