The present study describes a cell-based biosensor utilizing PEDOT electrodes coated with Nafion and polylysine for combined conductivity, cellular adhesion and proliferation. Neuroblastoma N2a cells were seeded on top of PEDOT electrodes treated with Nafion and Polylysine. Cellular attachment and viability were assayed and chronoamperometric measurements were taken to evaluate H202 toxicity. Cells exhibited relatively hight viability compared to those seeded in tissue culture plates. Chronoamperometric responses also provided preliminary evidence of the possible use of this assembly as a toxicity biosensor.
