Research ArticleTwo Groups of eGFP-Expressing Neurons with Distinct Characteristics in the Neocortex of GIN Mice
Section snippets
INTRODUCTION
GABAergic interneurons are one of two major types of neurons in the neocortex, which provide inhibition for neuronal networks and play crucial roles in the brain functions (Douglas and Martin, 2004). They are remarkably heterogeneous, consisting of many subtypes with distinct molecular profiles, morphologies, and intrinsic and synaptic properties (Markram et al., 2004, Rudy et al., 2011). Most cortical interneurons are generated in the medial ganglionic eminence and the caudal ganglionic
Transgenic Mice
Homozygous transgenic mice (FVB-Tg (GadGFP) 45704Swn/J, GIN mice) were purchased from the Jackson labs (Bar Harbor, ME) and offsprings of both sexes were bred in-house and used for experiments. A total of 134 mice were used in this study. All animals were maintained on 12-h light/dark cycles and were provided food and water ad libitum. All procedures used in the study adhered to the guidelines approved by the Institutional Animal Care and Use Committee at the University of Florida and the
Two Groups of eGFP+ Neurons in the Neocortex of GIN Mice
We examined the distribution of eGFP+ neurons in the neocortex of GIN mice aged P1 to P60. In addition to eGFP+ neurons distributed in the layers II-IV and upper layer V of the neocortex (cortical eGFP neurons), we observed massive eGFP+ neurons in the deep layers of the lateral neocortex (cluster eGFP neurons, Fig. 1A, indicated by arrows). These neurons have not been studied previously. In the present work, we examined the distributions, molecular markers, intrinsic and synaptic properties of
DISCUSSION
In the present study, we observed two groups of eGFP+ neurons in the neocortex of GIN mice, cluster eGFP neurons and cortical eGFP neurons. Cortical eGFP neurons, which populate cortical layers II-IV and upper layer V and contain SST, have been extensively studied previously (Halabisky et al., 2006, Chen et al., 2009, Okaty et al., 2009, Fanselow and Connors, 2010, Kinnischtzke et al., 2012, Pan et al., 2016, Riedemann et al., 2016, Schmid et al., 2016). Cluster eGFP neurons, however, have not
Acknowledgments
We thank Drs. Liu Junhua, Su Mingzhao and Chen Dongsheng for assistance with histology and immunohistochemistry. We also thank Dr. Wang Jinhui for help in part of experiments. Funding was provided by the Southwest University of China (5330500236) to HX Chen and National Institute of Neurological Disorders and Stroke Grant NS-35651 to S. N. Roper.
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