Elsevier

Neuroscience

Volume 170, Issue 2, 13 October 2010, Pages 497-502
Neuroscience

Cellular and Molecular Neuroscience
Research Paper
Olfactory type G-protein α subunit in striosome-matrix dopamine systems in adult mice

https://doi.org/10.1016/j.neuroscience.2010.06.072Get rights and content

Abstract

There is a growing body of evidence that striosome-matrix dopamine systems are tightly linked with motor and behavioral brain functions and disorders. In this study, we used an immunohistochemical method to show differential expression of the olfactory type G-protein α subunit (Gαolf) that involves in the coupling of dopamine D1 receptor with adenylyl cyclase in the striatal compartments of adult mice, and observed heightened density of Gαolf labeling in the striosomes relative to the matrix compartment. Our findings suggest that Gαolf could be one of the key molecules for controlling differential responses of the striosome and matrix compartments to dopamine D1 receptor signaling in the striatum of adult mice.

Section snippets

Experimental procedures

All procedures involving the use of animals and analysis of brain anatomy were approved by the Institutional Care and Use Committees of Tokushima University.

Results

To test whether the striosome and matrix compartments differentially respond to dopaminergic stimulation in the adult mice used in the present study, we performed an assay for c-Fos expression after treatment with apomorphine (Fig. 1). Because the induction of immediate early gene reflects acute elevation of cAMP-dependent signaling caused by the activation of postsynaptic D1Rs within striatal neurons, c-Fos induction can be considered as an indicator of D1R-mediated signal transduction in the

Discussion

Given that c-Fos induction by apomorphine is significantly high in the striosomes relative to the matrix compartment, our results indicate that there exists a predominant responsiveness of the striosome compartment to D1R signaling in the adult mouse striatum. However, according to our data, no apparent compartmentalization was found in adult mice with respect to the distribution of D1R or TH. This finding is consistent with the known expression pattern of D1R and TH in the mouse striatum (Kim

Acknowledgments

This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (grant-in-aid for Scientific Research, 20591025). We express our sincere appreciation to Professor Ann M Graybiel, Massachusetts Institute of Technology, for her helpful comments on this work.

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