Cellular and Molecular NeuroscienceResearch PaperOlfactory type G-protein α subunit in striosome-matrix dopamine systems in adult mice
Section snippets
Experimental procedures
All procedures involving the use of animals and analysis of brain anatomy were approved by the Institutional Care and Use Committees of Tokushima University.
Results
To test whether the striosome and matrix compartments differentially respond to dopaminergic stimulation in the adult mice used in the present study, we performed an assay for c-Fos expression after treatment with apomorphine (Fig. 1). Because the induction of immediate early gene reflects acute elevation of cAMP-dependent signaling caused by the activation of postsynaptic D1Rs within striatal neurons, c-Fos induction can be considered as an indicator of D1R-mediated signal transduction in the
Discussion
Given that c-Fos induction by apomorphine is significantly high in the striosomes relative to the matrix compartment, our results indicate that there exists a predominant responsiveness of the striosome compartment to D1R signaling in the adult mouse striatum. However, according to our data, no apparent compartmentalization was found in adult mice with respect to the distribution of D1R or TH. This finding is consistent with the known expression pattern of D1R and TH in the mouse striatum (Kim
Acknowledgments
This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (grant-in-aid for Scientific Research, 20591025). We express our sincere appreciation to Professor Ann M Graybiel, Massachusetts Institute of Technology, for her helpful comments on this work.
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